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板栗叶片是一种多糖和次级混合量均较高的植物材料,DNA提取的关键步骤是使用十六烷基三甲基溴化铵(CTAB)和聚乙烯吡咯啉酮(PVP)。经本文所描述的方法得到的DNA,完全能满足随机扩增多态性DNA(RAPD)分析。
Chestnut leaves is a plant material with high polysaccharide and secondary mixing. The key step in DNA extraction is cetyltrimethylammonium bromide (CTAB) and polyvinylpyrrolidone (PVP). The DNA obtained by the method described in this article can fully meet the random amplified polymorphic DNA (RAPD) analysis.