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目的研究人类疱疹病毒6型(HHV-6)、VEGF-C与CD44表达与口腔鳞状细胞癌颈部淋巴结转移的相关性及其临床意义。方法运用免疫组化检测40例口腔鳞状细胞癌、21例癌前病变及26例正常对照组组织中HHV-6、VEGF-C与CD44表达情况。结果正常对照组、癌前病变组、口腔鳞状细胞癌组组织中VEGF-C检出率分别为0、85.7%、90%,组间比较具统计学差异(P<0.05)。三组中HHV-6检出率分别为0、85.7%、87.5%,3组间HHV-6检出率逐渐提高趋势,组间比较提示统计学有显著性差异(P<0.005)。CD44在三组中的阳性表达率分别是100%、57.1%、12.5%,随着口腔粘膜上皮异型增生和癌前病变至鳞状细胞癌呈现表达逐渐下降之势,组间比较提示统计学有显著性差异(P<0.005)。同时研究表明,口腔鳞状细胞癌组VEGF-C和HHV-6表达与颈淋巴结转移有相关性,而CD44表达与口腔鳞状细胞癌颈淋巴结转移无相关性。结论 HHV-6感染、VEGF-C过度表达和CD44的表达下降可能参与了口腔粘膜上皮细胞异型增生并癌变的过程,与口腔鳞状细胞癌的发生存在相关性。HHV-6感染和VEGF-C过度表达与口腔鳞状细胞癌颈淋巴结转移有相关性。
Objective To study the correlation between the expression of HHV-6, VEGF-C and CD44 and cervical lymph node metastasis in oral squamous cell carcinoma and its clinical significance. Methods The expression of HHV-6, VEGF-C and CD44 in 40 cases of oral squamous cell carcinoma, 21 cases of precancerous lesions and 26 cases of normal control tissues were detected by immunohistochemistry. Results The positive rates of VEGF-C in normal control group, precancerous lesions group and oral squamous cell carcinoma group were 0, 85.7% and 90%, respectively. There was significant difference between two groups (P <0.05). The detection rates of HHV-6 in the three groups were 0, 85.7% and 87.5%, respectively. The detection rate of HHV-6 gradually increased among the three groups. Statistical analysis showed significant differences (P <0.005). The positive expression rates of CD44 in three groups were 100%, 57.1% and 12.5% respectively. With the progression of oral mucosal dysplasia and precancerous lesions to squamous cell carcinoma, the expression of CD44 gradually decreased. Significant difference (P <0.005). At the same time, it has been found that the expression of VEGF-C and HHV-6 in oral squamous cell carcinoma is correlated with lymph node metastasis, while the expression of CD44 is not correlated with cervical lymph node metastasis in oral squamous cell carcinoma. Conclusions HHV-6 infection, VEGF-C overexpression and decreased expression of CD44 may be involved in the process of dysplasia and carcinogenesis of oral mucosal epithelial cells, which is correlated with the occurrence of oral squamous cell carcinoma. HHV-6 infection and VEGF-C overexpression are associated with cervical lymph node metastasis in oral squamous cell carcinoma.