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采用28号(0.5时)针灸针1支和直径约0.5厘米的球形泡沫海绵小球一个.海绵穿在距针尖1厘米处.供测菌株接种在蔡培氏液体培养基内,在25℃下振荡培养2天,置4℃冰库内冷藏备用.接种时,先将海绵球蘸上分生孢子液,然后按针尖与棉苗茎成30°夹角,刺入棉苗子叶节.这时将针柄向棉苗茎杆挤压,使泡沫塑料内的分生孢子液(约0.02毫升)沿着针尖流入棉苗茎内的输导组织.拔出针尖,重蘸孢子液后进行第二株棉苗的接种.
A No. 28 (0.5) acupuncture needles and a diameter of about 0.5 cm spherical foam sponge beads a sponge wear at a distance of 1 cm from the needle tip for the test strains were inoculated in Cai Pei’s liquid medium at 25 ℃ Oscillatory culture for 2 days, set at 4 ℃ freezer cold reserve. Inoculation, first sponge ball dipped in conidia solution, and then press the tip of cotton seedlings into a 30 ° angle between stems, piercing cotton seedling leaf section. The needles are pressed against the stem of the cotton seedling so that the conidial fluid (about 0.02 ml) in the foam flows into the guiding tissue in the stem of the cotton seedling along the tip of the needle. Seedling cotton seedlings inoculation.