论文部分内容阅读
目的通过研究早期胚胎可溶性人类白细胞抗原G(sHLA-G)的表达,探讨sHLA-G在移植时选择理想胚胎的意义。方法应用酶联免疫吸附实验(ELISA)定量检测体外培养2 d胚胎培养液中sHLA-G浓度。结果①60例胚胎培养液中sHLA-G阳性率为36.6%(22/60),其中妊娠组58.3%(14/24),未妊娠组22.2%(8/36);两者相比,差异有统计学意义(P<0.05);②胚胎培养液中平均sHLA-G浓度,妊娠组(2.0±0.8)U/ml,未妊娠组(1.2±1.0)U/ml;两者相比,差异有统计学意义(P<0.05);③双原核受精卵发育形成的胚胎sHLA-G阳性率为36.6%,与未受精卵子阳性率(6.25%,1/16)相比,P<0.05,差异有统计学意义;与多原核受精卵发育形成的胚胎阳性率(25%,4/16)相比,P>0.05,差异无统计学意义。结论早期细胞期胚胎有sHLA-G表达,其浓度高低与胚胎发育及种植有关,可作为选择理想移植胚胎的重要依据。
Objective To investigate the significance of sHLA-G in selecting ideal embryo during transplantation by studying the expression of soluble human leukocyte antigen G (sHLA-G) in early embryo. Methods The concentration of sHLA-G in embryo culture medium was detected by enzyme-linked immunosorbent assay (ELISA). Results ① The positive rate of sHLA-G in 60 cases of embryo culture was 36.6% (22/60), of which 58.3% (14/24) in pregnant group and 22.2% (8/36) in non-pregnant group. There was significant difference between the two groups The mean sHLA-G concentration in embryo culture medium was (2.0 ± 0.8) U / ml in pregnant group and (1.2 ± 1.0) U / ml in non-pregnant group. There was significant difference between the two groups (P <0.05). ③ The positive rate of sHLA-G in embryos formed by the development of dioprogenuclear fertilized eggs was 36.6%, which was significantly different from that of unfertilized oocytes (6.25%, 1/16) Statistical significance; Compared with the positive rate of embryo formed by the development of prokaryotic fertilized eggs (25%, 4/16), P> 0.05, the difference was not statistically significant. Conclusion The expression of sHLA-G in early stage cell embryos is closely related to embryonic development and implantation, which may serve as an important basis for selecting ideal embryos.