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报道了流感病毒体外诱导狗肾细胞系（ＭＤＣＫ）细胞凋亡的检测结果，对黑色素选择性抑制流感病毒诱导细胞凋亡的可能性进行了探讨，同时与临床上常用的抗病毒药物病毒唑的效果进行比较。结果显示：病毒感染６ｈ后，即可观测到宿主细胞核固缩现象、ＤＮＡ凝胶电泳出现特征性的梯状图谱，感染１２ｈ后，细胞核可见明显的裂解；并且流感病毒株Ａ１／京防８６１诱导细胞凋亡能力强于Ｂ沪防／９３－１；在２０～１２５μｇ／ｍＬ浓度范围内，黑色素可有效抑制６４个血凝单位（ＨＵ）的流感病毒感染诱导的细胞凋亡而无细胞毒性作用，其抑制效率类似病毒唑。初步研究结果表明：黑色素抗流感病毒诱导细胞凋亡机理与其阻断病毒吸附侵入宿主细胞有关
This paper reports the detection of apoptosis induced by influenza virus in canine kidney cell line (MDCK) in vitro and explores the possibility of melanin selectively inhibiting the apoptosis induced by influenza virus. At the same time, it has been compared with the commonly used antiviral drug ribavirin The effect is compared. The results showed that after 6h of virus infection, the nuclear condensation phenomenon of the host cell was observed. The characteristic ladder pattern appeared on the DNA gel electrophoresis. After 12h of infection, the cell nuclei showed obvious lysis; and the influenza virus A1 / 1 induced apoptosis more strongly than B / A / 93-1; melanin effectively inhibited influenza A virus-induced apoptosis in 64 hemagglutination units (HU) at a concentration of 20-125μg / mL without cells The toxic effect, its inhibitory efficiency similar to ribavirin. Preliminary results show that: melanoma anti-influenza virus-induced apoptosis mechanism and its blocking virus invasion and invasion of host cells