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采用水培方法,分析了5个粳稻品种(系)在正常和低氮2种氮量水平下的7个氮素代谢指标和3个农艺指标的差异。结果表明:与正常施氮量处理相比,在低氮条件下,生产上表现耐瘠的滇杂35的根系氧化力、谷氨酰胺合成酶(GS)活性及叶绿素a、b和叶绿素总含量及有效穗数均没有显著变化,但根系还原力显著上升,硝酸还原酶(NR)活性显著下降,株高及生物产量极显著下降;其余4个供试粳稻品种(系)的根系还原力没有显著上升,但根系氧化力等9个指标值均发生了显著或极显著变化,这说明耐低氮胁迫的品种具有根系还原力显著上升,而根系氧化力、谷氨酰胺合成酶(GS)活性、叶绿素b、叶绿素总含量和有效穗数变化不明显的特性。这些特性可以作为耐低氮水稻材料筛选的参考指标。
The hydroponics method was used to analyze the differences of seven nitrogen metabolism indexes and three agronomic indexes of five japonica cultivars (lines) under the two nitrogen levels of normal and low nitrogen. The results showed that compared with the normal nitrogen application, the root oxidation, glutamine synthetase (GS) activity and total chlorophyll a, b and total chlorophyll content of Dianza 35, And effective panicle number did not change significantly, but the root reduction power increased significantly, nitrate reductase (NR) activity decreased significantly, plant height and biological yield decreased significantly; the other four tested japonica rice varieties (lines) (P <0.05). However, all the nine indexes such as root oxidative stress had significant or extremely significant changes, which indicated that the root restitution ability of the varieties with low nitrogen stress increased significantly, while the root oxidative stress, glutamine synthetase (GS) activity , Chlorophyll b, total content of chlorophyll and effective panicle changes were not obvious characteristics. These characteristics can be used as a reference index for screening low nitrogen-resistant rice materials.