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目的通过对HBV转基因小鼠CD4+、CD4+CD25+调节性T细胞数量和免疫抑制功能的研究,探讨CD4+CD25+调节性T细胞在乙肝免疫耐受中的作用。方法用流式细胞术对12只HBV转基因小鼠和12只正常小鼠外周血CD4+、CD4+CD25+调节性T细胞的频率进行检测;磁珠分选小鼠脾CD4+CD25-T细胞和CD4+CD25+T细胞,分为HBsAg刺激组和ConA刺激组体外单独和共培养,ELISA方法检测诱生的细胞因子IL-2。结果与正常小鼠比较,HBV转基因小鼠外周血CD4+、CD4+CD25+调节性T细胞数量差异无统计学意义(P>0.05)。HBsAg刺激组,CD4+CD25-T细胞单独或共培养,HBV转基因小鼠CD4+CD25-T细胞诱生IL-2水平显著低于正常小鼠(P<0.01);ConA刺激组,HBV转基因小鼠CD4+CD25-T细胞诱生IL-2水平与正常小鼠相比则差异无统计学意义(P>0.05);两组小鼠CD4+CD25-T细胞单独培养时诱生IL-2水平均显著高于共培养(P<0.01)。结论与正常小鼠比较,HBV转基因小鼠CD4+、CD4+CD25+调节性T细胞数量和免疫抑制功能差异无统计学意义,但T细胞水平对乙肝病毒存在特异性免疫耐受。CD4+CD25+调节性T细胞可抑制CD4+、CD8+T细胞。
OBJECTIVE: To investigate the role of CD4 + CD25 + regulatory T cells in immune tolerance of hepatitis B (HBV) by studying the number of CD4 +, CD4 + CD25 + regulatory T cells and immunosuppressive function in HBV transgenic mice. Methods The frequencies of CD4 +, CD4 + CD25 + regulatory T cells in 12 HBV transgenic mice and 12 normal mice were detected by flow cytometry. The spleen CD4 + CD25-T cells and CD4 + CD25 + T cells were divided into HBsAg stimulation group and ConA stimulation group alone and in co-culture, ELISA method to detect the induced cytokine IL-2. Results Compared with normal mice, there was no significant difference in CD4 +, CD4 + CD25 + regulatory T cells in peripheral blood of HBV transgenic mice (P> 0.05). The level of IL-2 in HBsAg-stimulated group and CD4 + CD25-T cells was significantly lower than that in normal mice (P <0.01) There was no significant difference in the levels of IL-2 induced by CD4 + CD25-T cells between normal mice and mice (P> 0.05). IL-2 levels were induced in CD4 + CD25-T cells Were significantly higher than co-culture (P <0.01). Conclusion Compared with normal mice, the number of CD4 +, CD4 + CD25 + regulatory T cells and the immunosuppressive function in HBV transgenic mice were not significantly different. However, T cells had specific immune tolerance to hepatitis B virus. CD4 + CD25 + regulatory T cells can inhibit CD4 +, CD8 + T cells.