论文部分内容阅读
目的：探讨口腔粘膜上皮细胞的培养扩增方法及生物学特性。方法：取手术中切除的多余粘膜，分别用胰酶和Ｄｉｓｐａｓｅ分离后经胰酶消化，接种无血清培养基。将二者细胞数量、活力及融合时间加以比较，同时对细胞各代增值特点、生长曲线、克隆形成率及血清对细胞分化的影响进行观察。结果：Ｄｉｓｐａｓｅ分离法的细胞总数，活细胞数均高于胰酶分离法。粘膜上皮细胞能够在无血清培养基稳定增殖传代，血清对上皮细胞有诱导分化作用。结论：Ｄｉｓｐａｓｅ分离的无血清培养技术可短期内获得大量具增殖能力的粘膜上皮细胞。
Objective: To investigate the method of culture and biological characterization of oral mucosa epithelial cells. Methods: To remove the excess mucosal resection, were separated by trypsin and Dispase trypsin digestion, seeding serum-free medium. The cell number, vitality and fusion time of the two cells were compared, and the value-added characteristics, growth curve, clonogenic rate and the effect of serum on cell differentiation were observed. Results: The total number of cells and the number of viable cells in Dispase separation method were higher than that of trypsin separation method. Mucosal epithelial cells can be stably propagated in serum-free medium, and serum can induce differentiation of epithelial cells. CONCLUSIONS: Dispase-free, serum-free culture can provide large numbers of proliferative mucosal epithelial cells in a short period of time.