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本文介绍一种新的液体培养系统,当外源性刺激因素存在时,允许红系集落生长和人骨髓细胞增殖数周。培养器包括由二根不锈钢管作成的内扩散室(15×11毫米).置于培养皿中(90×15毫米)。内扩散室的外管内侧有管闸固定内管,其下端有三足以便培养液通过紧密固定在内管底部的透析膜。两管顶端敞开供气体交换和倒装显微镜直接观察。内扩散室经高压消毒后装入包含2×10~5有核细胞
This article describes a new liquid culture system that allows red colony growth and proliferation of human bone marrow cells for several weeks when exogenous stimuli are present. The incubator consisted of an internal diffusion chamber (15 x 11 mm) made of two stainless steel tubes and placed in petri dishes (90 x 15 mm). Inside the inner tube of the inner diffusion chamber, a tube brake is used to fix the inner tube, and the lower end of the inner tube is provided with a three-legged dialysis membrane for tightly fixing the culture fluid to the bottom of the inner tube. The top of the two tubes is open for gas exchange and inverted microscope observation. Internal diffusion chamber after autoclave containing 2 × 10 ~ 5 nucleated cells