论文部分内容阅读
利用ISSR(inter simplesequencerepeats)技术比较了 2 4份中国半冬性、瑞典冬性和瑞典春性油菜的遗传多样性。 2 0个引物扩增出了 12 5条多态性带。UPGMA聚类分析将 2 4份材料分为 3组。第一组为 6份瑞典冬性和 8份中国半冬性材料 ,第二组为 2个中国半冬性材料湘油 15号和保 81,第三组为 8份瑞典春性材料。主成分分析结果与聚类分析结果相似 ,表明研究所用中国半冬性、瑞典冬性和瑞典春性 3类材料彼此间区分明显 ,中国半冬性油菜与瑞典冬性油菜的遗传关系比与瑞典春性油菜的关系近。结果显示 ,ISSR技术是估计油菜种质资源遗传多样性的有效手段
The genetic diversity of twenty-four Chinese winter, Swedish winter and Swedish spring rape were compared using the ISSR (inter simple sequence repeats) technique. Twenty-five polymorphic bands were amplified with 20 primers. UPGMA cluster analysis divided 24 materials into 3 groups. The first group consisted of 6 Swedish winter and 8 Chinese semi-winter materials, the second group 2 Chinese semi-winter materials Xiangyou 15 and Bao 81, and the third group 8 Swedish Swedish spring materials. The result of principal component analysis is similar to the results of cluster analysis. It shows that the semi-winter in China, winter in Sweden and Spring in Sweden are obviously distinguished from each other. The genetic relationship between Chinese semi-winter canola and Swedish winter canola is better than that in Sweden Relationship between spring rape. The results showed that ISSR technique was an effective method to estimate the genetic diversity of rape germplasm resources