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成功的精子和卵子相互作用取决于精子获能,而其获能状态则由顶体反应和精子活动力所确定。作者从三个不同方面评价了配子的相互作用:(1)结合到透明带的精子数量与精子浓度和孵化时间的关系。(2)精子与透明带结合过程中的获能状况和精子顶体反应的动力学。(3)人的卵泡液对不同人精子与透明带结合能力和精子顶体反应状态的影响。
Successful sperm-egg interactions depend on sperm capacitation, whereas their capacitances are determined by acrosome reaction and sperm motility. The authors evaluated the gamete interactions in three different ways: (1) the number of sperm bound to the zona pellucida versus sperm concentration and incubation time. (2) The kinetics of capacitation and sperm acrosome reaction during sperm-zona pellucida binding. (3) The effect of human follicular fluid on the binding ability of different human sperm and zona pellucida and sperm acrosome reaction state.