PURPOSE: To evaluate the clinical applicability of a novel scanning laser ophthalmoscopy imaging system for ultra-wide-field fluorescein angiography. DESIGN: Observational case report. METHODS: Wide-field fluorescein angiography images were captured in normal volunteers and in patients with a variety of clinical disorders with a newly developed scanning laser ophthalmoscope (Optomap; Optos PLC., Dunfermline, Scotland, United Kingdom) that produces high-resolution images of the fundus up to a 200-degree field. Fluorescein angiograms were performed using standard sequence times. RESULTS: Peripheral vascular perfusion of all four quadrants of a normal fundus was observed with good detail of the capillary architecture. Patients with diabetes and ocular in-flammatory eye disease showed evidence of peripheral retinal nonperfusion. The relative degrees of retinal perfusion and nonperfusion could be compared in single images. CONCLUSIONS: Ultra-wide-field fundus fluorescein angiography offers the possibility of evaluating and quantitating peripheral retinal perfusion and vascular pathology in fundus disease. METHODS: Wide-field fluorescein angiography images were captured in normal volunteers and in patients with a variety of clinical disorders with a newly developed scanning laser ophthalmoscope (Optomap; Optos PLC., Dunfermline, Scotland, United Kingdom) that produces high-resolution images of the fundus up to a 200-degree field. Fluorescein angiograms were performed using standard sequence times. : Peripheral vascular perfusion of all four quadrants of a normal fundus was observed with good detail of the capillary architecture. Patients with diabetes and ocular in-flammatory eye disease showed evidence of peripheral retinal nonperfusion. The relative degrees of retinal perfusion and nonperfusion could be more in single images. CONCLUSIONS: Ultra-wide-field fundus fluorescein angiography offers the possibility of evaluating and quantitating peripheral retinal perfusion and vascular pathology in fundus disease.