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目的 观察妊娠高血压综合征 (PIH)孕妇外周血及其脐血姐妹染色单体互换 (SCE)频率 ,探讨PIH的病因以提高PIH的检测水平。方法 采用姐妹染色单体互换 (SCE)法和放免法分别对 2 1例PIH病人和 15例正常孕妇 (对照组 )的外周血及其所分娩新生儿脐血SCE频率的测定。结果 PIH组外周血和脐血SCE频率分别为 (6 11± 1 2 9)次 ,(5 98± 1 38)次 ;对照组分别为 (3 45± 0 71)次 ,(3 16± 0 5 7)次。其两组比较 ,差异有极显著性 (P <0 0 1)。PIH组外周血和脐血SCE频率皆异常。结论 PIH与遗传DNA物质损伤有关 ,SCE频率可作为一项诊断、预测PIH的重要指标。
Objective To observe the frequency of sister chromatid exchange (SCE) in peripheral blood of pregnant women with pregnancy induced hypertension (PIH) and their umbilical cord blood and explore the etiology of PIH to improve the detection of PIH. Methods SCE and radioimmunoassay were used to determine the frequency of SCE in peripheral blood of 21 PIH patients and 15 normal pregnant women (control group), respectively. Results The frequency of SCE in peripheral blood and umbilical cord blood of PIH group was (6 11 ± 1 2 9) times and (5 98 ± 1 38) times respectively, while the control group was (3 45 ± 0 71) times and (3 16 ± 0 5) 7) times. The difference between the two groups was significant (P <0.01). PIH group peripheral blood and cord blood SCE frequency are abnormal. Conclusion PIH is related to genetic DNA damage, and SCE frequency can be used as an important index for diagnosis and prediction of PIH.