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目的采用125I粒子对胰腺癌细胞Sw1990及Panc-1行体外持续照射,研究其生物学效应,探讨连续照射对胰腺癌细胞增殖、DNA合成、细胞周期及凋亡的影响,并为胰腺癌放射实验细胞的选择提供参考。方法将胰腺癌细胞Sw1990及Panc-1体外培养至对数生长期,行125I粒子持续照射,在初始剂量率为12.13 cGy/h时,分别给予总剂量为0、2、4、6、8 Gy的照射,采用克隆形成实验检测照射后细胞的增殖能力,绘制生存曲线,计算细胞存活率(SF2),检测细胞凋亡率和细胞周期,以及3H-TDR掺入实验探究照射后细胞DNA合成情况。结果经过拟合,计算出Sw1990和Panc-1细胞的SF2值分别为0.766±0.063和0.729±0.045,随着照射剂量增高,两种细胞凋亡率也逐渐升高,Panc-1细胞的最大凋亡率出现在6 Gy,Sw1990出现在8 Gy。G2/M期阻滞分数均逐渐升高,3H-TDR掺入放射量逐渐降低。结论125I持续照射胰腺癌细胞时,细胞凋亡及G2/M期阻滞是抑制细胞增殖的主要原因,在剂量为0、2、4、6、8 Gy时,Sw1990及Panc-1细胞生物学效应差异无统计学意义。
OBJECTIVE: To study the biological effects of 125I particles in vitro on human pancreatic cancer cells (SW1990 and Panc-1) in vitro and to investigate the effects of continuous irradiation on the proliferation, DNA synthesis, cell cycle and apoptosis of pancreatic cancer cells. Cell selection for reference. Methods The pancreatic cancer cells, Sw1990 and Panc-1, were cultured in vitro until the logarithmic growth phase. 125 I seeds were irradiated continuously. When the initial dose was 12.13 cGy / h, the total dose of 0, 2, 4, 6, 8 Gy The proliferative ability of the cells after irradiation was measured by clone formation assay. The survival curves were plotted, the cell viability (SF2) was calculated, the apoptosis rate and cell cycle were detected, and 3H-TDR incorporation assay was used to explore the DNA synthesis after irradiation . Results After fitting, the SF2 values of Sw1990 and Panc-1 cells were calculated as 0.766 ± 0.063 and 0.729 ± 0.045, respectively. With the increase of radiation dose, the apoptosis rates of the two kinds of cells also increased gradually. The maximum apoptosis of Panc-1 cells Mortality occurred at 6 Gy, with Sw1990 at 8 Gy. G2 / M phase arrest scores were gradually increased, 3H-TDR incorporation of radioactivity gradually decreased. CONCLUSION: The apoptosis of human pancreatic cancer cell line 125I and G2 / M phase arrest are the main reasons for the inhibition of cell proliferation. At the doses of 0, 2, 4, 6 and 8 Gy, the cell biology of Sw1990 and Panc-1 cells No significant difference in effect.