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目的大鼠在非麻醉状态下,采用双尾静脉穿刺术实施高胰岛素-正血糖钳夹实验并与局麻下尾动静脉插管高胰岛素-正血糖钳夹实验进行比较。方法普通饲料喂养和高脂高糖饲料喂养的SD大鼠各8只,喂养8周后,各分为2组,每组4只,分别进行不同的高胰岛素-正血糖钳夹实验;大鼠在非麻醉状态下,选用两条尾静脉行静脉穿刺,一条尾静脉靠近根部输入胰岛素[0.12 U/(kg·h)]和葡萄糖,同时另一条尾静脉靠近尾末1/3采血,进行高胰岛素-正血糖钳夹实验。结果大鼠在非麻醉状态下双尾静脉穿刺术后血糖在正常范围内,无明显波动,无大鼠死亡;高脂高糖饲料喂养组大鼠的葡萄糖输注率显著低于对照组(P<0.01);血糖变异系数和葡萄糖输入速率变异系数分别为5.7%和14.9%;双尾静脉穿刺术高胰岛素-正血糖钳夹实验与尾动静脉插管术高胰岛素-正血糖钳夹实验两组结果之间差异无统计学意义。结论在非麻醉状态下采用双尾静脉穿刺术行大鼠的高胰岛素-正血糖钳夹实验,具有安全可行、实验结果稳定、准确、操作简便、实验周期短、术后大鼠恢复快、可多次重复进行等优点;大鼠饲喂高脂高糖饲料可诱发明显的胰岛素抵抗。
In the non-anesthetized state, the rats were subjected to hyperinsulinemic-noglycemic clamp by two-tail vein puncture and compared with the tail vein-cannulated hyperinsulinemic-noglycemic clamp under local anesthesia. Methods Eight SD rats fed with normal diet and high-fat and high-sucrose diet were divided into two groups with 4 rats in each group after 8 weeks of feeding. Different high-insulin-orthophylolipidemic clamp experiments were performed. Rats In the non-anesthetic state, two tail veins were used for venipuncture. One tail vein was infused with insulin [0.12 U / (kg · h)] and glucose near the root, while the other tail vena - Positive blood glucose clamp experiment. Results In non-anesthetized rats, the blood glucose level was within the normal range after two-tail vein puncture without any significant fluctuations and the rats died. The glucose infusion rate in rats fed with high-fat and high-sugar diet was significantly lower than that in the control group <0.01). The coefficient of variation (CV) and glucose input rate (CV) were 5.7% and 14.9%, respectively. Two-tail vein apheresis hyperinsulinemic-nogasular clamp and tail arteriovenous cannulation hyperinsulinemic- No significant difference between the group results. Conclusions It is safe and feasible to perform hyperinsulinemic-orthoglycidal clamp in rats under the condition of non-anesthesia by two-tail vein puncture. The experimental results are stable, accurate, simple and convenient, the experimental period is short, and the rats recover quickly. Repeated multiple advantages; rats fed high-fat, high-sugar diet can induce significant insulin resistance.