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目的应用双重免疫组化染色法,研究P53与细胞角蛋白在鼻咽癌中的表达。方法50例鼻咽部低分化鳞癌组织,P53单克隆抗体,CK多克隆抗体,双重免疫组化染色。结果50例鼻咽部低分化鳞癌组织标本均有不同数量的双标记阳性细胞,即P53与CK同时表达。结论双重免疫组化染色法,可用于研究P53与细胞角蛋白在鼻咽癌中的表达。
Objective To study the expression of P53 and cytokeratin in nasopharyngeal carcinoma by double immunohistochemistry. Methods 50 cases of nasopharyngeal poorly differentiated squamous cell carcinoma, P53 monoclonal antibody, CK polyclonal antibody, double immunohistochemical staining. Results 50 cases of nasopharyngeal poorly differentiated squamous cell carcinoma specimens have a different number of double-labeled positive cells, that P53 and CK expression. Conclusions Double immunohistochemical staining can be used to study the expression of P53 and cytokeratin in nasopharyngeal carcinoma.