论文部分内容阅读
目的研究超声波、温度与过氧乙酸的协同杀菌作用,为医疗器械消毒实际应用提供依据。方法采用载体浸泡定量和定性杀菌试验方法,对超声波等物理因子与过氧乙酸协同杀菌效果进行了试验研究。结果用过氧乙酸750 mg/L单独浸泡作用4 min,对载体上枯草杆菌黑色变种芽孢的平均杀灭率为99.93%;在超声波水槽内含350 mg/L过氧乙酸水溶液加温50℃超声波的协同作用2 min,对载体上枯草芽孢杆菌黑色变种芽孢的杀灭率达100%。用含1 500 mg/L过氧乙酸单独浸泡作用15 min,对载体上枯草杆菌黑色变种芽孢定性培养达到无菌生长;在超声波水槽内含750 mg/L过氧乙酸水溶液加温50℃超声波的协同作用10 min,对载体上枯草杆菌黑色变种芽孢定性培养达到无菌生长。结论超声波加温50℃与过氧乙酸协同作用,可明显提高过氧乙酸杀灭细菌芽孢的效果,对其用于临床医疗器械消毒具有参考价值。
Objective To study the synergistic bactericidal effect of ultrasound, temperature and peracetic acid to provide the basis for the practical application of medical device disinfection. Methods The method of carrier immersion quantitative and qualitative sterilization was used to study the synergistic bactericidal effect of physical factors such as ultrasound and peracetic acid. Results The average killing rate of Bacillus subtilis black spores on the carrier was 99.93% with 750 mg / L peracetic acid alone for 4 min. The ultrasonic wave was stirred at 350 ℃ for 60 min in a water bath containing 350 mg / L peracetic acid Synergistic effect for 2 min, the killing rate of B. subtilis black variant spores on the carrier reached 100%. With 15 500 mg / L peracetic acid alone soaking 15 min, the carrier on the Bacillus subtilis qualitative transformation of bacteria to achieve aseptic growth; ultrasonic bath containing 750 mg / L peracetic acid solution heated 50 ℃ ultrasound Synergistic effect of 10 min, the carrier of Bacillus subtilis qualitative transformation of Bacillus aseptic growth. Conclusion The synergistic effect of ultrasonic heating at 50 ℃ and peracetic acid can obviously improve the effect of peracetic acid in killing bacterial spores, which is of reference value for the disinfection of clinical medical instruments.