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研究人外周血T淋巴细胞中白细胞介素2(IL-2)mRNA的表达和分泌IL-2的活力,实验结果表明植物血凝素(PHA)单独诱导IL-2的能力很弱,诱导过程较长;加入促癌剂12-0-十四酰佛波-13-乙酯(TPA)后能诱导IL-2的早期转录,并增强16h后PHA诱导的转录作用和培养细胞的上层液中IL-2的活力。45℃,10min的热休克不影响IL-2的诱导合成。在分析、比较PHA,TPA和/或热休克影响的基础上,对T淋巴细胞激活过程中IL-2基因表达的调控机制进行了讨论。
To study the expression of interleukin 2 (IL-2) mRNA in peripheral blood T lymphocytes and the activity of IL-2 secretion, the results show that phytohaemagglutinin (PHA) has a weak ability of inducing IL-2 alone. The induction process Was longer than that of control group. The addition of the promoter of 12-0-tetradecanoylphosphato-13-ethyl ester (TPA) induced the early transcription of IL-2 and enhanced the PHA-induced transcription and the supernatant of cultured cells after 16 h IL-2 activity. Heat shock at 45 ° C for 10 min did not affect IL-2 induced synthesis. Based on the analysis and comparison of the effects of PHA, TPA and / or heat shock, the regulatory mechanism of IL-2 gene expression during T lymphocyte activation was discussed.