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食用菌的育种及其遗传、变异研究都需要进行单孢子分离。单孢子的分离方法很多,如琼脂平板稀释分离法、玻璃毛细管分离法、显微操作器分离法等,但这些方法都有各自的特点,而且各人的实验条件不同其效果也不一样。本文介绍一种简单、实用、快速、准确的单孢分离方法,具体操作步骤如下:
Edible fungi breeding and genetic, mutation studies need to carry out single spore separation. There are many methods for the isolation of monospores, such as agar plate dilution method, glass capillary separation method, micromanipulator separation method, etc. However, these methods have their own characteristics, and each of the different experimental conditions are not the same effect. This article describes a simple, practical, rapid and accurate single spore separation method, the specific steps are as follows: