论文部分内容阅读
目的探讨病原相关模式分子(PAMPs)对抗体诱导的关节炎(CAIA)样小鼠模型建立的影响。方法 7~8周龄Balb/c雌鼠,第0天使用抗体混合物诱导,第3天使用LPS(TLR4激动剂)或Cp G ODN 1826(TLR9激动剂)激发,每天定时观察四肢关节发病情况并进行关节评分;小鼠腹腔注射O111:B4和O55:B5,绘制生存曲线进行毒性分析;ELISA检测PAMPs刺激小鼠巨噬细胞后IL-6、TNF-α的分泌水平;鲎实验定量检测相同浓度下O111:B4和O55:B5的有效活性。结果 PAMPs成功激发CAIA模型炎症,其中O55:B5低死亡率、高成模率;O111:B4的毒性强于O55:B5;O55:B5的炎症因子分泌量显著高于O111:B4和Cp G ODN 1826(P<0.01);O111:B4的有效活性显著高于O55:B5(P<0.01)。结论低毒高致炎性的O55:B5激发模型效果最佳,说明PAMPs能够影响建模效果,而低毒高致炎性PAMPs适合该模型的激发。
Objective To investigate the effects of pathogen-associated pattern molecules (PAMPs) on the establishment of antibody-induced arthritis (CAIA) -like mouse models. Methods Balb / c female mice aged 7-8 weeks were induced with antibody mixture on day 0 and challenged with LPS (TLR4 agonist) or CpG ODN 1826 (TLR9 agonist) on day 3, and the incidence of limb joints was observed daily The mice were intraperitoneally injected with O111: B4 and O55: B5, and the survival curves were drawn for toxicity analysis. The levels of IL-6 and TNF-α secreted by PAMPs in macrophages were detected by ELISA. Effective activity of O111: B4 and O55: B5 under. Results PAMPs successfully induced inflammation in CAIA model, in which O55: B5 low mortality rate and high modulus rate; O111: B4 was more toxic than O55: B5; O55: B5 had higher secretion of inflammatory cytokines than O111: B4 and CpG ODN1826 P <0.01). The effective activity of O111: B4 was significantly higher than that of O55: B5 (P <0.01). Conclusion The model of O55: B5 with low toxicity and high inflammation has the best effect, indicating that PAMPs can affect the modeling effect, while low toxicity and high inflammatory PAMPs are suitable for the model.