目的观察参麦的抗休克效果,探讨其作用机制。方法大鼠随机分为实验对照组(control),双击组(HS+LPS),参麦注射液组(HS+LPS+SM)。测定血清中NO含量,NOS活性以及肝脏κB及TIMP1mRNAI的表达。结果HS+LPS+SM组NOS活性,NO2/NO3含量显著低于LPS组(P<0.05),HS+LPS+SM组IκB及TIMP1mRNA表达明显增加。结论参麦可能是通过影响NOS活性降低NO的含量,并上调肝脏组织中IκB和TIMP1mRNA的表达,减轻内毒素引起的机体损伤。 Objective To observe the anti-shock effect of Shenmai and explore its mechanism of action. Methods Rats were randomly divided into experimental control group, double-click group (HS+LPS) and Shenmai injection group (HS+LPS+SM). Serum NO levels, NOS activity, and expression of hepatic κB and TIMP1 mRNAI were determined. Results The activity of NOS and NO2/NO3 in HS+LPS+SM group were significantly lower than those in LPS group (P<0.05). The expression of IκB and TIMP1 mRNA in HS+LPS+SM group was significantly increased. Conclusion Shenmai may reduce the NO content by affecting the activity of NOS, and up-regulate the expression of IκB and TIMP1 mRNA in liver tissue and reduce the body damage caused by endotoxin.