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以往工作发现，中脑导水管周围灰质（ＰＡＧ）和海马在非阿片肽ＡＣＴＨ痛觉调制中占有重要地位。但它们之间的相互影响在ＡＣＴＨ痛觉调制中的作用，尚未阐明。本工作利用免疫组织化学和病阈测定方法，进一步观察大鼠ＰＡＧ和海马在ＡＣＴＨ镇痛时的相互关系，并与吗啡镇痛作用相比较。结果如下：（１）海马内注射ＡＣＩＨ（０．５ｕ／４μｌ）或吗啡（５μｇ／４μｌ），痛阈明显升高（１１９．３±４．７％，１２２．７±２６．８％）与对照组比较有显著差异（Ｐ＜０．０１），该明显效应均可被ＰＡＧ内注射阿片受体拮抗剂纳络酮所阻断；（２）ＰＡＧ内注射吗啡或ＡＣＴＨ后病阈提高更显著（１８０．９±５０．３％，２１９．８±７７．０％，Ｐ＜０．０１），但海马内注射纳络酮可阻断前者的效应（Ｐ＜０．０５）而对后者却无影响（Ｐ＞０．０５）；（３）伤害性刺激福尔马林（Ｆ）可诱发大鼠脊髓腰膨大背角原癌基因ｃ－ｆｏｓ显著表达，以Ⅰ、Ⅱ层较显著，海马或ＰＡＧ内注射ＡＣＴＨ均可抑制其背角ｃ－ｆｏｓ表达。结果提示：ＰＡＧ、海马均参与ＡＣＴＨ和阿片系统对脊髓痛信息传递的调制作用，在ＡＣＴＨ痛觉调制作用中，表明ＰＡＧ、海马之间的相互影响是复杂的。
Previous work found that periaqueductal gray matter (PAG) and hippocampus play an important role in the modulation of non-opioid ACTH pain. However, the interaction between them in ACTH pain modulation has not been elucidated yet. This work using immunohistochemistry and disease threshold measurement method to further observe the rat PAG and hippocampus in ACTH analgesia correlation and morphine analgesia compared. The results were as follows: (1) The pain threshold was significantly increased (119.3 ± 4.7%, 122.7 ± 26.8%) in the hippocampus with ACIH (0.5u / 4μl) or morphine (5μg / (P <0.01), and the obvious effect was blocked by the intra-PAG injection of naloxone, an opioid receptor antagonist. (2) The threshold of the disease after the injection of morphine or ACTH in PAG increased more significantly (180.9 ± 50.3%, 219.8 ± 77.0%, P <0.01). However, the injection of naloxone in the hippocampus blocked the effect of the former (P <0.05) But no effect (P> 0.05). (3) The nociceptive stimulation of formalin significantly induced the expression of proto-oncogene c-fos in the lumbar dorsal horn of lumbar spinal cord in rats, ACTH injection into the hippocampus or PAG inhibited the expression of c-fos in the dorsal horn. The results suggest that both PAG and hippocampus are involved in the modulatory effect of ACTH and opioid on the transmission of spinal pain information. In the pain modulation of ACTH, it shows that the interaction between PAG and hippocampus is complex.