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目的 制备大肠杆菌 (E .coli)核心型单一特异性抗血清 ,建立E .coli核心型血清学检测法 ,检测致泻性E .coli核心型。方法 ELISA间接法 ,用吸收后单一核心型特异性抗血清检测鉴定E .coli核心型。结果 (1)吸收后获得单一核心型特异性抗血清 ,对E .coli核心型的检测结果与相应单克隆抗体检测结果完全相同 ;(2 )经鉴定表明 ,吸收后单一核心型特异性抗血清识别结合的抗原成分既非E .coli核酸和蛋白 ,又非E .coli类脂A和内核心KDO 庚糖 ,而是构成E .coli核心型的外核心己糖物质 ;(3)四类 2 7株不同血清型致泻性E .coli核心型检测结果为 :R1核心型 15株 (5 5 .5 % )、R3核心型 10株 (37.1% ) ,K12核心型 2株 (7.4% ) ,未检测出R2和R4核心型。结论 吸收后E .coli核心型抗血清具有较高单一核心型特异性 ,可用于E .coli核心型的检测。
Objective To prepare E.coli core single antiserum and establish E.coli core serological test to detect diarrheal E.coli core type. Methods ELISA indirect method, with a single type of core after absorption specific antiserum detection identified E.coli core type. Results (1) Single core type specific antiserum was obtained after absorption, and the results of core strain of E.coli were exactly the same as those of the corresponding monoclonal antibodies. (2) After identification, single core type specific antiserum The recognized antigenic component is neither E. coli nucleic acids nor the protein, nor E. coli lipid A and the inner core KDO heptose, but instead constitutes the outer core heparin of E. coli core type; (3) Seven strains of different serotypes of diarrhea e. Coli core type test results were: R1 core type 15 (55.5%), R3 core type 10 (37.1%), K12 core type 2 (7.4%), Not detected R2 and R4 core type. Conclusion After absorption, E.coli core antiserum has higher single core specificity and can be used for the detection of E.coli core type.