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目的研究小干扰RNA-siRNA对人甲状腺癌SW579细胞STAT3表达及细胞增殖和侵袭的抑制作用。方法将STAT3-siRNA转染给人甲状腺癌SW579细胞来沉默STAT3的表达。用逆转录聚合酶链式反应(RT-PCR)和Western blotting测定STAT3mRNA和蛋白的表达。用甲基噻唑基四唑(MTT)来分析STAT3STAT3-siRNA对癌细胞的生长抑制作用。通过RT-PCR检测MMP2和MMP9mR-NA的表达,Transwell侵袭小室实验研究癌细胞的体外侵袭能力。结果STAT3-siRNA能有效而稳定地抑制甲状腺癌SW579中STAT3的表达,下调STAT3可以在体外显著抑制肿瘤细胞的生长,在转染后24、48、72h,转染STAT3特异性的siRNA组生长抑制率显著高于对照组。siRNA沉默STAT3基因后,肿瘤细胞的体外侵袭能力明显下降。结论下调STAT3可以在体外显著抑制肿瘤细胞的生长和侵袭能力,STAT3特异性siRNA作为一种治疗肿瘤的新途径值得进一步研究。
Objective To study the inhibitory effect of small interfering RNA-siRNA on STAT3 expression and cell proliferation and invasion in human thyroid carcinoma SW579 cells. Methods STAT3-siRNA was transfected into human thyroid carcinoma SW579 cells to silence STAT3 expression. STAT3 mRNA and protein expression were determined by reverse transcription polymerase chain reaction (RT-PCR) and Western blotting. Methylthiazolyltetrazole (MTT) was used to analyze the growth inhibitory effect of STAT3STAT3-siRNA on cancer cells. The expression of MMP2 and MMP9mR-NA was detected by RT-PCR. Transwell invasive chamber assay was used to investigate the in vitro invasion ability of cancer cells. Results STAT3-siRNA could effectively and stably inhibit the expression of STAT3 in thyroid cancer SW579 cells. Down-regulation of STAT3 could significantly inhibit the growth of tumor cells in vitro. At 24, 48 and 72 h after transfection, the STAT3-siRNA-transfected STAT3- The rate was significantly higher than the control group. After silencing STAT3 by siRNA, the invasion ability of tumor cells in vitro was significantly decreased. Conclusion Down-regulation of STAT3 can significantly inhibit the growth and invasion ability of tumor cells in vitro. STAT3-specific siRNA as a new way to treat tumors deserves further study.