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目的 探讨卡托普利（ＣＡＰ）对家兔肾上腹主动脉阻断致急性肾缺血／再灌注损伤（ＡＲＩＲＩ）的保护效果。方法 于肾上阻断腹主动脉血流３０ｍｉｎ再灌注１８０ｍｉｎ的方法制成双侧ＡＲＩＲＩ动物模型，将２４只家兔随机等分为假手术组（Ａ组）、缺血再灌注组（Ｂ组）和ＣＡＰ治疗组（Ｃ组）。Ｃ组于阻断主动脉前 ５ｍｉｎ静注 ＣＡＰ２ｍｇ· ｋｇ~（－１），继之持续输注 １５ｍｉｎ ＣＡＰ ０．５ｍｇ· ｋｇ~（－１）· ｈ~（－１）。 Ａ、Ｂ组以等容积生理盐水取代ＣＡＰ，Ａ组不阻断主动脉血流。动态检测血中ＢＵＮ、Ｃｒ、ＳＯＤ、ＭＤＡ、ＡＴ－Ⅱ，尿β_２－ＭＧ变化，以及肾皮质中ＳＯＤ、ＭＤＡ、ＡＴ－Ⅱ和肾组织形态学改变。结果 Ｃ组在再灌期血和肾皮质中ＭＤＡ、ＡＴ－Ⅱ浓度明显低于Ｂ组（Ｐ＜０．０１），ＳＯＤ活性显著高于Ｂ组（Ｐ＜０．０１），血ＢＵＮ、Ｃｒ及尿中β２－ＭＧ含量明显低于Ｂ组（Ｐ＜０．０５，＜０．０１）。Ｃ组光镜下肾小管损伤Ｐａｌｌｅｒ评分明显低于Ｂ组（２０．５０±７．５６ｖｓ ８２．５０±１６．６９，Ｐ＜０．０５），Ｂ组电镜见明显急性肾小管损伤及坏死，而Ｃ组肾小管损伤轻微。结论ＣＡＰ对家兔肾上腹主动脉阻断所致ＡＲＩＲＩ有良好的保护?
Objective To investigate the protective effect of captopril on acute renal ischemia / reperfusion injury (ARIRI) induced by the block of the abdominal aorta in rabbits. Methods The bilateral ARIRI animal models were established by blocking the abdominal aorta blood flow for 30 minutes and reperfused for 180 minutes. The 24 rabbits were randomly divided into sham operation group (A group), ischemia reperfusion group (B group) ) And CAP treatment group (C group). In group C, CAP 2 mg · kg -1 was intravenously injected 5 min before occlusion of the aorta followed by continuous infusion of 0.5 mg · kg -1 · h -1 CAP for 15 min. A, B group to replace the CAP volume of normal saline, A group does not block the aortic blood flow. The changes of BUN, Cr, SOD, MDA, AT-II and urinary β2-MG in the blood and the changes of SOD, MDA, AT-Ⅱ and renal tissue in renal cortex were detected dynamically. Results The concentrations of MDA and AT-Ⅱ in blood and renal cortex in group C were significantly lower than those in group B (P <0.01), and the activity of SOD in group C was significantly higher than that in group B (P <0.01) And urinary β2-MG content was significantly lower than the B group (P <0.05, <0.01). In Group C, the Paller score of renal tubular injury was significantly lower than that of Group B (20.50 ± 7.56 vs 82.50 ± 16.69, P <0.05). In group B, acute renal tubular injury and necrosis were observed, In group C, renal tubular injury was mild. Conclusion CAP has good protection on ARIRI induced by the blockage of the abdominal aorta in rabbits.