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目的研究人乳头状瘤病毒(HPV)E6、E7作用位点的各种细胞周期蛋白在宫颈癌的表达情况。方法选择73例HPV16阳性的宫颈鳞癌癌组织标本,其中35例来自澳大利亚,38例来自中国。采用半定量的免疫组化方法检测p53、pRb、p16INK4A、p21CIP1/WAF1、p27KIP1和细胞周期素D1(cyclin D1)的表达情况。结果两组患者在平均年龄、淋巴结转移、肿瘤细胞分化程度方面的差异无统计学意义,但中国组较澳大利亚组进展期宫颈癌(>Ⅱa期)患者为多(P=0.007)。澳大利亚组宫颈癌组织p53的阳性表达率及pRb、p21和p27的上调率分别为3%、56%、63%和34%,中国组分别为23%、89%、97%和89%,两组癌组织中上述四种细胞周期蛋白的表达情况差异有统计学意义。p16和cyclin D1的上调率在澳大利亚组分别为97%和3%,在中国组分别为和100%和12%,两组比较差异无统计学意义。两组资料合并,p53总的阳性表达率为13%,pRb、p16、p21、p27和cyclinD1总的上调率分别为74%、99%、81%、63%和7%。结论HPV16阳性的宫颈癌明显存在pRb、p16、p21和p27的过度表达,而中国组宫颈癌p53阳性表达率和pRb、p21、p27的过度表达率比澳大利亚组明显增高,这提示HPV诱导宫颈癌的分子学途径复杂,有必要在不同人种进行进一步研究。
Objective To study the expression of various cyclin in human cervical cancer with human papillomavirus (HPV) E6 and E7 loci. Methods Totally 73 cases of HPV16 positive cervical squamous cell carcinoma specimens were selected, of which 35 cases were from Australia and 38 cases were from China. The expression of p53, pRb, p16INK4A, p21CIP1 / WAF1, p27KIP1 and cyclin D1 were detected by semi-quantitative immunohistochemistry. Results There were no significant differences in mean age, lymph node metastasis and tumor cell differentiation between the two groups. However, there were more cases in Chinese group than in advanced stage cervical cancer (> Ⅱa stage) in Australia (P = 0.007). The positive expression rates of p53 and pRb, p21 and p27 in cervical cancer tissues in Australia were 3%, 56%, 63% and 34% respectively, while those in Chinese group were 23%, 89%, 97% and 89% The expression of the above four cyclin in the group of cancer tissues had statistical significance. The up-regulation rates of p16 and cyclin D1 were 97% and 3% respectively in Australia and 100% and 12% respectively in China. There was no significant difference between the two groups. The two groups of data combined, the total positive rate of p53 was 13%, pRb, p16, p21, p27 and cyclinD1 total up-regulation rates were 74%, 99%, 81%, 63% and 7%. Conclusion The HPV16-positive cervical cancer has obvious over-expression of pRb, p16, p21 and p27, but the positive rate of p53 and the overexpression of pRb, p21 and p27 in cervical cancer in China are significantly higher than that in Australia, which indicates that HPV induces cervical cancer The complex molecular pathways, it is necessary to further study in different ethnic groups.