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本试验采用不同剂量的γ射线、电子束、NaN_3与 EMS 处理3个小麦品种,在总剂量不变的情况下,用γ射线间歇辐照;用育种常用剂量(250～300Gy)辐照16个品种和12个杂种,诱发小麦抗白粉病突变,在 M_2进行苗期抗性突变体的筛选。结果表明,丁射线、电子束、NaN_3与 EMS 均为诱发小麦抗白粉病突变的有效诱变剂,而且电子束与 NaN_3的诱变效果更好。不同诱变剂诱发白粉病抗性的适宜剂量:丫射线300～350GV,电子束100～200Gy,NaN_31～3mmol/L,EMS 0.3%左右,品种间略有差异。γ射线间歇辐照较连续辐照的突变频率高,杂合材料较纯合材料的诱变效果更好。利用核辐射获得86份抗白粉病的中间材料。
In this experiment, three wheat cultivars were treated with different doses of γ-ray, electron beam, NaN_3 and EMS, and irradiated intermittently with γ-rays at the same total dose. Sixteen irradiation doses of 250-300 Gy Varieties and 12 hybrids were used to induce wheat powdery mildew resistance mutations. M_2 was screened for resistant mutant at seedling stage. The results showed that Ding ray, electron beam, NaN_3 and EMS were all effective mutagens to induce wheat powdery mildew resistance mutation, and the effect of electron beam and NaN_3 mutagenesis was better. Different mutagens induced powdery mildew resistance appropriate dose: gamma ray 300 ~ 350GV, electron beam 100 ~ 200Gy, NaN_31 ~ 3mmol / L, EMS 0.3%, a slight difference between varieties. The γ-ray intermittent irradiation has a higher frequency of mutation than the continuous irradiation, and the hybrid material has better mutagenic effect than the homozygous material. Eighty-eight powdery mildew resistant intermediate materials were obtained using nuclear irradiation.