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采用双向电泳和MALDI-TOF-TOF质谱联用技术,对近年来河南省的新生黄叶病害杨树进行了差异蛋白质组学研究。经图谱分析,在叶片、韧皮部和根部分别获得了98个(61个上调,37个下调)、90个(42个上调,48个下调)和41个(19个上调,22个下调)差异蛋白点。对其中差异表达1.5倍以上的61个蛋白点进行了质谱鉴定,58个蛋白质点鉴定成功,这些蛋白点可归类于26种蛋白质。这些蛋白包括参与光合作用和与抗性相关的蛋白。研究结果有助于揭示病害的发生机理,铁蛋白的鉴定结果对病因的确定具有重要参考价值。
Two-dimensional electrophoresis and MALDI-TOF-TOF mass spectrometry were used to study differential proteomics of poplar in Henan Province with yellow leaf disease in recent years. According to the phylogenetic analysis, 98 (61 up-regulated and 37 down-regulated), 90 (42 up-regulated and 48 down-regulated) and 41 (19 up-regulated and down-regulated) differential proteins point. Sixty-six protein spots were differentially expressed more than 1.5 times, and 58 protein spots were successfully identified. These spots were classified as 26 kinds of proteins. These proteins include proteins involved in photosynthesis and resistance. The results will help reveal the mechanism of disease occurrence, ferritin identification results of the etiology of the important reference value.