本文基于３，４－二羟基苯甲酸对Ｃｏ２＋离子催化的鲁米诺－Ｈ２Ｏ２化学发光体系的抑制作用，首次建立了３，４－二羟基苯甲酸的流动注射化学发光抑制分析法，发光信号的降低值ΔⅠ与３，４－二羟基苯甲酸的浓度在１×１０－６～１×１０－５ｍｏｌ／Ｌ的范围内呈良好的线性关系，检出限为２．７×１０－７ｍｏｌ／Ｌ（ｎ＝１１），对８×１０－６ｍｏｌ／Ｌ３，４－二羟基苯甲酸测定的相对标准偏差为２．３％（ｎ＝１１）。 Based on the inhibitory effect of 3,4-dihydroxybenzoic acid on the luminol-H2O2 chemiluminescence system catalyzed by Co2 + ions, flow-injection chemiluminescence inhibition assay of 3,4-dihydroxybenzoic acid was established for the first time. Decreasing values ​​of ΔI and 3,4-dihydroxybenzoic acid showed good linearity in the range of 1 × 10-6 ~ 1 × 10-5mol / L with a detection limit of 2.7 × 10-7mol / L (N = 11) and a relative standard deviation of 2.3% (n = 11) for the measurement of 8 × 10 -6 mol / L 3,4-dihydroxybenzoic acid.