目的:探讨促红细胞生成素(EPO)对哮喘模型小鼠肺部炎症改变及细胞因子的影响。方法:将60只BALB/c雄性小鼠随机分为哮喘模型组(B组)、哮喘+EPO低浓度组(C组)、哮喘+EPO中浓度组(D组)、哮喘+EPO高浓度组(E组)、地塞米松组(F组)和正常对照组(A组),每组10只,各组小鼠用组胺行支气管激发试验。测定每组小鼠气道阻力,检测气道反应性;采用HE染色观察肺组织病理形态学改变特征,利用酶联免疫吸附试验(ELISA)法测定血清中免疫球蛋白E(Ig E)、白细胞介素-6(IL-6)、白细胞介素-10(IL-10)、肿瘤坏死因子-α(TNF-α)的变化。结果:1气道反应性:B组小鼠的气道阻力较A组增高,差异有统计学意义(P<0.05)。D、E、F组气道阻力较B组明显降低,差异有统计学意义(P<0.05)。2Ig E:B组小鼠血清Ig E的浓度较A组明显增高,差异有统计学意义(P<0.01);D、E、F组动物血清中Ig E低于B组,差异有统计学意义(P<0.01),C组Ig E高于F组,差异有统计学意义(P<0.01)。3IL-6和TNF-α:B组小鼠血清IL-6和TNF-α水平较A组明显增高,差异有统计学意义(P<0.05或P<0.01);D、E、F组IL-6和TNF-α水平明显低于B组,差异有统计学意义(P<0.05或P<0.01)。C组IL-6和TNF-α水平高于F组,差异有统计学意义(P<0.05或P<0.01),D、E组IL-6和TNF-α水平高于F组,但差异无统计学意义(P>0.05)。4IL-10:B组IL-10的水平较A组明显下降,差异有统计学意义(P<0.01);D、E、F组IL-10水平明显高于B组,差异有统计学意义(P<0.05)。5肺组织病理改变:B组小鼠肺部血管及气管周围大量炎症细胞浸润及水肿,基底膜明显增厚,基底层平滑肌增生;C、D、E、F组小鼠肺部血管及气道周围炎症细胞逐渐减少及基底层逐渐变薄,较B组明显减轻。结论:EPO可减轻哮喘模型小鼠肺部炎症改变及减轻气道反应性。 Objective: To investigate the effect of erythropoietin (EPO) on lung inflammation and cytokines in asthmatic mice. Methods: Sixty BALB / c male mice were randomly divided into asthma model group (group B), asthma + EPO low concentration group (group C), asthma + EPO concentration group (group D), asthma + EPO high concentration group (Group E), dexamethasone group (group F), and normal control group (group A). ​​Each group was given bronchoalveolar stimulation with histamine. The airway resistance of each group was measured and the airway responsiveness was measured. The histopathological features of the lung tissue were observed by HE staining. The levels of IgE and leukocyte in the serum were measured by enzyme linked immunosorbent assay (ELISA) (IL-6), interleukin-10 (IL-10) and tumor necrosis factor-α (TNF-α) Results: 1 Airway reactivity: The airway resistance of group B was higher than that of group A, the difference was statistically significant (P <0.05). The resistance of airway in group D, E and F was significantly lower than that in group B, the difference was statistically significant (P <0.05). IgE in group B was significantly higher than that in group A (P <0.01); IgE in serum of group D, E and F was lower than that in group B, the difference was statistically significant (P <0.01). The Ig E in group C was higher than that in group F, the difference was statistically significant (P <0.01). The levels of IL-6 and TNF-α in 3IL-6 and TNF-α: B groups were significantly higher than those in A group (P <0.05 or P <0.01) 6 and TNF-α in group B were significantly lower than those in group B (P <0.05 or P <0.01). The levels of IL-6 and TNF-α in group C were significantly higher than those in group F (P <0.05 or P <0.01), while the levels of IL-6 and TNF-α in group D and E were higher than those in group F Statistical significance (P> 0.05). The levels of IL-10 in 4IL-10: B group were significantly lower than those in A group (P <0.01), and the levels of IL-10 in D, E and F groups were significantly higher than those in B group P <0.05). Pathological changes in lung tissue: A large number of inflammatory cell infiltration and edema around the blood vessels and trachea in group B mice were significantly thicker basement membrane and basal smooth muscle hyperplasia; lung blood vessels and airways of mice in groups C, D, E and F Inflammatory cells around the gradual reduction and basal layer gradually thinning, significantly reduced compared with the B group. Conclusion: EPO can reduce lung inflammation and reduce airway responsiveness in asthmatic mice.