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目的:建立参附汤中人参皂苷Rg1、Re、Rb1的含量测定方法。方法:采用Inertsil ODS–SP(4.6mm×250mm,5μm)色谱柱,柱温25℃,流动相为乙腈:水,梯度洗脱,流速:1.0mL.min-1,检测波长203nm。结果:人参皂苷Rg1在12.5～500μg.mL-1线性关系良好,r=0.9999,平均回收率98.86%,RSD为2.32%;人参皂苷Re在12.5～500μg.mL-1线性关系良好,r=0.9999,平均回收率99.09%,RSD为2.22%;人参皂苷Rb1在12.5～500μg.mL-1线性关系良好,r=0.9999,平均回收率99.53%,RSD为1.68%;结论:该方法学考察符合定量要求,结果准确,可用于同时测定参附汤中人参皂苷Rg1,Re,Rb1的含量,为参附汤的质量控制提供依据。
Objective: To establish a method for the determination of ginsenoside Rg1, Re, Rb1 in Shenfu Decoction. Methods: Inertsil ODS-SP (4.6mm × 250mm, 5μm) column was used. The column temperature was 25 ℃. The mobile phase consisted of acetonitrile and water with a gradient of 1.0 mL · min-1. The detection wavelength was 203 nm. Results: The linear relationship of ginsenoside Rg1 was 12.5 ~ 500μg.mL-1, r = 0.9999, the average recovery was 98.86%, RSD was 2.32%. The ginsenoside Re was good at 12.5 ~ 500μg.mL-1, r = 0.9999 , The average recovery was 99.09% and the RSD was 2.22%. The linear relationship of ginsenoside Rb1 was 12.5 ~ 500μg.mL-1, r = 0.9999, the average recovery was 99.53% and the RSD was 1.68% .Conclusion: Requirements, the results are accurate, can be used for simultaneous determination of ginsenosides in ginseng soup ginsenoside Rg1, Re, Rb1 content, provide the basis for the quality control of Shen Fu Tang.