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目的:建立测定人血浆中头孢地尼含量的高效液相色谱(HPLC)方法。方法:血浆样品用高氯酸沉淀蛋白,采用Ulti-mate C18色谱柱(5μm,2 504.6mm),以乙腈:0.025mol.L-1磷酸二氢钠缓冲盐溶液(pH3.24)=15∶85作为流动相进行分离,检测波长为286nm,流速为1.0mL.min-1。结果:头孢地尼血药浓度30~2 500ng.mL-1范围内线性关系良好,最低检测浓度为30ng.mL-1。方法回收率(n=5)分别为76.41%,63.78%,66.44%,日内和日间精密度均小于15%。结论:该法操作简单、灵敏、准确、重现性好,适用于头孢地尼临床药代动力学研究。
Objective: To establish a high performance liquid chromatography (HPLC) method for the determination of cefdinir in human plasma. METHODS: Plasma samples were precipitated with perchloric acid and analyzed by Ulti-mate C18 column (5 μm, 2 504.6 mm) with acetonitrile: 0.025 mol·L-1 sodium phosphate monobasic phosphate buffered saline (pH 3.24) = 15: 85 as the mobile phase separation, detection wavelength of 286nm, a flow rate of 1.0mL.min-1. Results: The linear range of cefdinir was 30 ng · mL-1 and the lowest concentration was 30 ng · mL-1. The recoveries (n = 5) were 76.41%, 63.78% and 66.44%, respectively. The intra-and inter-day precision were less than 15%. Conclusion: The method is simple, sensitive, accurate and reproducible. It is suitable for cefdinir clinical pharmacokinetic studies.