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为了保障饮用牛奶的安全和人们健康,本研究对乌鲁木齐市2007～2010年共计47085头奶牛,采用结核菌素(PPD)皮内变态反应方法进行检疫,对检出的199头阳性牛养殖场(户)进行流行病学调查,同时用发达国家通用的比较变态反应和γ-干扰素实验进行比对实验,对阳性牛进行剖检,观察病理变化,采集病变肺脏和肺门淋巴结进行细菌培养和菌型鉴定。结果显示:国外比较变态反应与γ-干扰素检测具有较高的符合率;奶牛结核病分离到的牛型结核分枝杆菌占80%,人结核病分离到的人结核分枝杆菌占92.3%。乌鲁木齐市牛结核病的发病和流行与环境污染、活畜交易市场和集贸市场、畜及畜产品流通环节中的污染、饲养管理不科学、饲草料的污染、养殖人员自身疾病的影响和检疫净化的全面实施等相关。本实验的研究结果有利于乌鲁木齐奶牛结核病防治措施的制定和实施。
In order to ensure the safety of drinking milk and people’s health, a total of 47085 dairy cows in Urumqi from 2007 to 2010 were quarantined by the method of intradermal hypersensitive reaction of tuberculin (PPD). Among the 199 positive cow farms Households) epidemiological survey, at the same time with developed countries, the general comparison of allergic reactions and interferon-γ experiments, positive cattle were necropsy, pathological changes were observed, the lesion lung and hilar lymph nodes were collected for bacterial culture and Identification of bacteria. The results showed that there was a high coincidence rate of foreign allergic reaction with γ-interferon detection; Mycobacterium bovis isolated from cow tuberculosis accounted for 80%, and human Mycobacterium tuberculosis isolated from human tuberculosis accounted for 92.3%. Urumqi, the incidence and epidemic of bovine tuberculosis and environmental pollution, livestock market and bazaars, livestock and livestock products circulation links in the pollution, feeding and management unscientific, forage pollution, aquaculture personnel own disease and quarantine Purification of the full implementation of related. The results of this experiment are conducive to the formulation and implementation of prevention and treatment measures for tuberculosis in Urumqi dairy cows.