论文部分内容阅读
小麦(Triticum aestivum)品系贵州98-18对中国目前大多数叶锈菌(Puccinia triticina)生理小种表现抗性。基因推导表明,贵州98-18可能携带新的抗叶锈基因。为了有效利用这一抗源,将贵州98-18和感病小麦品种郑州5389杂交,获得F1、F2代群体,用我国叶锈菌优势小种THTT对双亲及其杂交后代进行接种鉴定。结果表明,贵州98-18对THTT的抗性由1对显性基因控制,暂命名为LrG98。采用SSR技术对贵州98-18携带的抗病基因进行分子标记,共筛选了1 274对SSR或STS引物,位于1BL染色体上的4对引物可在抗/感池和双亲中扩增出多态性DNA片段。遗传连锁分析结果表明,该抗病基因位于小麦1BL染色体上,与Xbarc582-1B和Lr26的STS标记ω-secali(Glu-B3)的遗传距离最近,均为3.8 cM。该基因与目前所有已知的抗叶锈基因不同,可能是1个新的抗病基因。
Triticum aestivum strain Guizhou 98-18 showed resistance to most current Chinese race of Puccinia triticina. Gene deduction shows that Guizhou 98-18 may carry new leaf rust resistance genes. In order to effectively use this resistance source, Guizhou 98-18 and susceptible wheat variety Zhengzhou 5389 were crossed to obtain F1 and F2 populations. The parents and their hybrid progenies were inoculated with THTT, the dominant species of leaf rust in our country. The results showed that the resistance of Guizhou 98-18 to THTT was controlled by a pair of dominant genes and was temporarily named LrG98. SSR was used to carry molecular markers of disease resistance genes carried by Guizhou 98-18. A total of 1 274 pairs of SSR or STS primers were screened. Four pairs of primers located on chromosome 1BL could amplify the polymorphisms in resistance / sense pool and parents Sex DNA fragments. Genetic linkage analysis showed that the resistance gene was located on chromosome 1BL of wheat and the genetic distance to ST-labeled ω-secali (Glu-B3) of Xbarc582-1B and Lr26 was 3.8 cM. This gene is different from all known anti-leaf rust genes and may be a new resistance gene.