论文部分内容阅读
目的探讨乙醇、甲苯、苯对骨髓造血干细胞和造血微环境的联合毒作用。方法乙醇、甲苯、苯对雌性ＢＡＬＢ／Ｃ小鼠染毒２７周，应用脾集落技术测定其多向造血干细胞和应用整体灌注固定原位电镜观察其骨髓造血微环境。结果 在染毒２～ ４个月时４０ｍｇ／ｍ~３苯合并自由饮用 １０％乙醇组较单纯同等剂量苯染毒对骨髓多向造血干细胞形成有促进作用，且 ＦＣＭ测定示其骨髓细胞合成期比例显著增加，并出现异倍体峰，提示乙醇增强了苯的诱癌作用；且对造血微环境的损伤作用明显增强，如晚幼粒细胞内含有髓样小体，并出现形态各异的特殊颗粒，针状包涵体，血窦窦壁不完整。结论高剂量苯暴露减低骨髓造血干细胞形成和损伤造血微环境。乙醇加强苯的毒性，而甲苯具一定保护作用。
Objective To investigate the combined toxic effects of ethanol, toluene and benzene on bone marrow hematopoietic stem cells and hematopoietic microenvironment. Methods Female BALB / C mice were exposed to alcohol, toluene and benzene for 27 weeks. Splenic colony assay was used to determine the multipotential hematopoietic stem cells. The bone marrow hematopoietic microenvironment was observed by using echocardiography. Results Compared with the same dose of benzoic acid, 40 mg / m 3 benzene and 10% ethanol were more effective in multipotential hematopoietic stem cell formation in 2-4 months after exposure, and FCM showed that the bone marrow cells were in the period of synthesis A significant increase in the proportion of aneuploidy peaks, suggesting that ethanol enhances the carcinogenic effect of benzene and hematopoietic microenvironment injury significantly enhanced, such as late promyelocytic containing medullary bodies, and the appearance of different forms Special particles, acicular inclusions, sinuses sinus wall is incomplete. Conclusion Exposure to high doses of benzene reduces bone marrow hematopoietic stem cell formation and damages the hematopoietic microenvironment. Ethanol enhances the toxicity of benzene, while toluene has a certain protective effect.