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背景:内皮素-1(endothelin-1,ET-1)和血管内皮细胞生长因子(vascularendothelialgrowthfactor,VEGF)在蛋白及核酸水平方面的研究多见于动物实验,而ET-1和VEGF与临床脑梗死病变方面关系的研究还未广泛开展。目的:探讨脑梗死发病中ET-1和VEGF的变化规律及机制。设计:完全随机对照研究。地点和对象:选自2000-03/06中国医科大学第二临床学院神经内科住院脑梗死患者27例及同期在本院正常体检老年人22例。干预:患者先行头CT或MRI检查,发病第3天及4周抽取肘静脉血,分别采用放免法及ELISA法测定血浆中ET-1和血清中VEGF含量。主要观察指标:血浆中ET-1和血清中VEGF含量。结果:脑梗死组ET-1及VEGF含量犤(65.7±10.8)ng/L,(419.5±176.3)ng/L犦均高于对照组犤(48.8±6.9)ng/L,(205.7±101.8)ng/L犦,两组比较差异有显著性意义(t=2.015,P<0.05,t=2.705,P<0.01);到恢复期时有所下降犤ET-1含量为(60.2±11.5)ng/L,VEGF含量为(309.4±138.9)ng/L犦,与对照组比较,差异有显著性意义(t=2.028,P<0.05;t=2.724,P<0.01)。ET-1及VEGF含量与梗死灶的大小及病情程度有关。结论:ET-1及VEGF与脑梗死的发生、发展密切相关,从而为脑卒中预防及康复干预提供实验依据。
BACKGROUND: Studies on the protein and nucleic acid levels of endothelin-1 (ET-1) and vascular endothelial growth factor (VEGF) have been found in animal experiments. ET-1, VEGF and clinical cerebral infarction Research on the relationship has not yet been widely carried out. Objective: To investigate the changes and mechanisms of ET-1 and VEGF in the pathogenesis of cerebral infarction. Design: Complete randomized controlled study. Location and Subjects: From March 2000 to June 06, 2006, 27 cases of in-hospital cerebral infarction in Department of Neurology of the Second Clinical College of China Medical University and 22 cases of normal elderly people in our hospital during the same period. Intervention: Patients were given head CT or MRI examinations, elbow venous blood was drawn on the 3rd and 4th week after the onset of disease, and the levels of plasma ET-1 and serum VEGF were determined by radioimmunoassay and ELISA respectively. MAIN OUTCOME MEASURES: Plasma ET-1 and serum VEGF levels. Results: The levels of ET-1 and VEGF in cerebral infarction group were significantly higher than those in control group (65.7 ± 10.8 ng / L, 419.5 ± 176.3 ng / L, 48.8 ± 6.9 ng / L, 205.7 ± 101.8, (t = 2.015, P <0.05, t = 2.705, P <0.01), and decreased to the recovery time (60.2 ± 11.5) ng / L, the difference between the two groups was significant / L, and the content of VEGF was (309.4 ± 138.9) ng / L 犦, which was significantly different from the control group (t = 2.028, P <0.05; t = 2.724, P <0.01). ET-1 and VEGF content and infarct size and severity of the disease. Conclusion: ET-1 and VEGF are closely related to the occurrence and development of cerebral infarction, and provide an experimental basis for stroke prevention and rehabilitation intervention.