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应用ISSR技术对 12个水稻光温敏核不育系进行DNA指纹分析 ,筛选到 13个多态性丰富的引物 ,共获得 16 9个多态性DNA片段 ,选择其中的 3个引物扩增出的 8个多态性DNA片段可作为鉴定标记 ,能够正确区分供试的 12个水稻光温敏核不育系。根据多态性片段的有无 ,将鉴定标记转换为数字 1和 0 ,建立了 12个水稻光温敏核不育系相应的ISSR标记鉴定识别码。由Nei’s遗传距离创建的聚类分析表明 ,12个材料被聚为两大类群 ,4个粳型不育系聚为一类 ,其余 8个籼型不育系聚为另一类 ,在籼稻群内 ,3个来源于安农S - 1的不育系单独聚为一类 ,与来源于农垦 5 8S的材料有明显的遗传差异。研究结果表明 ,ISSR标记具有简便、快速、多态性丰富等优点 ,可用于构建DNA指纹图谱 ,进行品种鉴定和遗传分析。
ISSR technique was used to analyze DNA fingerprinting of 12 TGMS lines. Thirteen primers with abundant polymorphism were screened out and 16 9 polymorphic DNA fragments were obtained. Three of them were selected for amplification Of the eight polymorphic DNA fragments can be used as identification markers, can correctly distinguish the 12 tested rice photo-thermo-sensitive genic male sterile lines. According to the presence or absence of polymorphic fragments, the identification markers were converted into numbers 1 and 0, and corresponding ISSR identification codes of 12 rice thermo-thermo-sensitive genic male sterile lines were established. Cluster analysis based on the Nei’s genetic distance showed that 12 materials were clustered into two groups, four japonica sterile lines clustered into one group and the remaining eight indica sterile lines clustered into another group. In indica rice Three CMS lines derived from Annong S - 1 were clustered together and had obvious genetic differences with those derived from Nongken 5 8S. The results showed that the ISSR marker has the advantages of simple, rapid, rich polymorphism, which can be used to construct DNA fingerprinting, identification and genetic analysis.