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目的探讨HOXa-10基因在小鼠胚胎着床过程中的作用。方法应用实时荧光定量PCR(FQ-PCR)分别检测未孕及早孕小鼠第1、2、3、4、57、d HOXa-10基因表达量的变化规律;同时用可以持续表达HOXa-10的DNA/脂质体复合物质粒和HOXa-10反义寡脱氧核糖核酸分别转染到受孕2d小鼠子宫角内,观察胚泡着床数。结果FQ-PCR结果显示,随着小鼠妊娠天数的增加,HOXa-10基因表达量也逐渐增高,在孕4d达到峰值,孕5d开始下降,孕7d时表达量已接近未孕状态;在用HOXa-10 DNA质粒/脂质体转染孕2d小鼠子宫角后,小鼠的胚泡着床数明显增加;用HOXa-10反义寡脱氧核糖核酸/脂质体转染孕2d小鼠子宫角后,小鼠的胚泡着床数明显减少(P<0.05)。结论在胚泡植入窗口期,子宫内膜HOXa-10基因表达上调,其可能参与了胚泡着床过程。
Objective To investigate the role of HOXa-10 gene in mouse embryo implantation. Methods The changes of gene expression of HOXa-10 at the 1st, 2nd, 3rd, 4th, 57th and the 90th day after pregnancy were detected by real-time fluorescence quantitative PCR (FQ-PCR) DNA / liposome complex plasmid and HOXa-10 antisense oligodeoxynucleotide were transfected into the uterine horn of pregnant mice 2d respectively to observe the number of blastocyst implantation. Results The FQ-PCR results showed that the HOXa-10 gene expression increased gradually with the increase of gestational days, reached its peak at 4 d, decreased at 5 d and reached the level of non-pregnant at 7 d. HOXa-10 DNA plasmid / liposome transfected 2h mouse uterine horn, the mouse blastocyst implantation significantly increased; with HOXa-10 antisense oligodeoxynucleotide / liposome transfected pregnant 2d mice After the uterine horn, the number of blastocyst implantation in mice was significantly reduced (P <0.05). Conclusion During the blastocyst implantation window, endometrial HOXa-10 gene expression is up-regulated, which may be involved in blastocyst implantation.