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背景与目的:近年来的研究表明,多种生物学指标与结直肠癌发生、发展以及手术后的复发转移、预后有关。本研究旨在探讨人结直肠癌组织中Survivin、MMP-2、nm23-H1、VEGF及其受体Flt-1的表达与各临床病理参数及预后的关系及临床意义。方法:采用免疫组织化学SP法,检测72例结直肠癌组织及其对照癌旁正常黏膜组织中Survivin、MMP-2、nm23-H1、VEGF及其受体Flt-1表达的状况,分析其与临床病理参数及复发转移的关系。结果:在结直肠癌中Survivin、MMP-2、nm23-H1、VEGF及其受体Flt-1的表达率分别为62.5%(45/72)、66.7%(48/72)、55.5%(40/72)、61.1%(44/72)、79.2%(57/72),均显著高于对照癌旁正常黏膜组织(P<0.01)。Survivin表达与浸润深度、淋巴结转移、TNM分期及术后复发转移有关;MMP-2表达与淋巴结转移、TNM分期、术后复发转移有关;nm23-H1表达与淋巴结转移、TNM分期相关;VEGF表达与浸润深度、TNM分期及术后复发转移有关;而受体Flt-1表达与临床病理及预后因素均无关。结论:Survivin、MMP-2、nm23-H1、VEGF与结直肠癌的发生、发展密切相关,联合检测多个相关基因的表达能更准确地判断结直肠癌的生物学特征及预后判断。
BACKGROUND AND PURPOSE: In recent years, studies have shown that a variety of biological indicators and colorectal cancer occurrence and development, as well as postoperative recurrence and metastasis, the prognosis. This study aimed to investigate the relationship between the expression of Survivin, MMP-2, nm23-H1, VEGF and their receptor Flt-1 in human colorectal cancer tissues and the clinicopathological parameters and prognosis. Methods: The expressions of Survivin, MMP-2, nm23-H1, VEGF and their receptor Flt-1 in 72 colorectal cancer tissues and their adjacent normal mucosa tissues were detected by immunohistochemical SP method. Clinical pathological parameters and recurrence and metastasis. Results: The expressions of Survivin, MMP-2, nm23-H1, VEGF and their receptor Flt-1 in colorectal cancer were 62.5% (45/72), 66.7% (48/72) and 55.5% /72),61.1%(44/72),79.2%(57/72), which were significantly higher than those in normal mucosa (P <0.01). The expression of Survivin was related to the depth of invasion, lymph node metastasis, TNM stage and recurrence and metastasis. The expression of MMP-2 was related to lymph node metastasis, TNM stage and postoperative recurrence and metastasis. The expression of nm23-H1 was correlated with lymph node metastasis and TNM stage. Depth of invasion, TNM stage and postoperative recurrence and metastasis, while the expression of Flt-1 receptor had no correlation with clinical pathology and prognosis. Conclusion: Survivin, MMP-2, nm23-H1 and VEGF are closely related to the occurrence and development of colorectal cancer. Combined detection of the expression of multiple related genes can determine the biological characteristics and prognosis of colorectal cancer more accurately.