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目的:应用荧光原位杂交技术(FISH)分析宫颈病变中宫颈脱落细胞的3号染色体hTERC基因异常情况,及其与HPV感染在宫颈癌中的关系。方法:对120例宫颈疾病患者,采用宫颈疾病三阶梯诊断方法,对正常宫颈(20例)、CIN、宫颈鳞癌、腺癌进行诊断,全面了解宫颈细胞形态学、组织病理学及HPV感染情况,并进行分型、分期,以病理检查为金标准;第2部分采用荧光原位杂交技术对其宫颈脱落细胞进行同步分析,了解3号染色体上hTERC基因扩增情况。第2部分采用反向杂交法检测HPV感染情况,对100例宫颈癌及癌前病变患者的宫颈脱落细胞进行HPVDNA的检测。结果:①100例中宫颈鳞癌30例、腺癌6例、CIN64例,FISH检测宫颈癌及癌前病变的hTERC基因在3号染色体长臂26.3区域高频扩增,发生率分别为100.0%、100.0%、76.1%。②100例宫颈疾病患者的HPV总阳性率71.0%。在75例hTERC基因扩增阳性的患者中,HPV阳性所占的比例为89.3%(67/75),而HPV阴性所占比例仅为10.6%(8/75)。结论:3号染色体上hTERC基因在宫颈癌和癌前病变患者的宫颈脱落细胞中有不同程度的扩增。
Objective: To analyze the abnormality of hTERC gene on chromosome 3 of cervical exfoliated cells in cervical lesions by fluorescence in situ hybridization (FISH) and its relationship with HPV infection in cervical cancer. Methods: A total of 120 cases of cervical diseases were diagnosed by cervical three-step diagnosis of cervical disease, including normal cervix (20 cases), CIN, cervical squamous cell carcinoma and adenocarcinoma. The morphology, histopathology and HPV infection of cervical cell were comprehensively understood , And the classification, staging, pathological examination as the gold standard; the second part of the fluorescence in situ hybridization of cervical exfoliated cells were synchronized analysis of chromosome 3 hTERC gene amplification. In the second part, HPV infection was detected by reverse hybridization and HPV DNA was detected in 100 cases of cervical cancer and precancerous lesions. Results: ①100 cases of cervical squamous cell carcinoma in 30 cases, adenocarcinoma in 6 cases, CIN 64 cases, FISH detection of cervical cancer and precancerous lesions of hTERC gene in chromosome 3 long arm 26.3 region high frequency amplification, the incidence rates were 100.0% 100.0%, 76.1%. ② 100 cases of cervical disease in patients with HPV positive rate of 71.0%. Among the 75 patients with positive hTERC gene amplification, HPV positive rate was 89.3% (67/75), while HPV negative rate was only 10.6% (8/75). Conclusion: The hTERC gene on chromosome 3 has different degrees of amplification in cervical exfoliated cells in patients with cervical cancer and precancerous lesions.