论文部分内容阅读
目的研究半胱氨酰白三烯受体1(CysLT1)在支气管哮喘(哮喘)大鼠肺组织中的表达,探讨炎性因子IFN-γ是否参与调节哮喘大鼠肺组织CysLT1的表达。方法以卵清蛋白致敏激发制作大鼠哮喘模型,然后测定不同质量浓度组胺时大鼠呼吸道压力,再取其肺组织,采用免疫组织化学法、反转录-聚合酶链反应法检测IFN-γ干预前后哮喘大鼠肺组织中CysLT1 mRNA的表达。结果当各实验组大鼠吸入组胺质量浓度为0 mg·L-1、10 mg·L-1和20 mg·L-1时,测得呼吸道阻力值,任意2组比较差异均有统计学意义(Pa<0.01);免疫组织化学(IHC)法检测正常对照组(N组)、哮喘模型组(A组)、哮喘鼠雾化吸入低剂量IFN-γ干预组(L组)及高剂量干预组(H组)的CysLTl阳性细胞表达计数分别为11.889±2.369、19.333±3.240、26.000±5.522、33.222±4.493,任意2组比较差异均有统计学意义(Pa<0.01);RT-PCR法检测N组、A组、L组及H组的CysLT1 mRNA/β-actin相对密度分别为0.218±0.050、0.323±0.084、0.447±0.083、0.670±0.108,任意2组比较差异均有统计学意义(Pa<0.01)。结论高、低剂量IFN-γ均能上调CysLT1在哮喘大鼠肺组织的表达,其中炎性因子IFN-γ可能通过上调CysLT1的表达水平而参与哮喘的发生发展。
Objective To investigate the expression of CysLT1 in the lung tissue of asthmatic rats and explore whether IFN-γ is involved in the regulation of CysLT1 expression in asthmatic rats. Methods The rat model of asthma was induced by ovalbumin sensitization. The airway pressure was measured in rats with different concentrations of histamine. The lung tissue was harvested and the expression of IFN was detected by immunohistochemistry and reverse transcription - polymerase chain reaction Expression of CysLT1 mRNA in the lung tissue of asthmatic rats before and after γ intervention. Results Respiratory resistance was measured when the inhaled histamine concentrations were 0 mg · L-1, 10 mg · L-1 and 20 mg · L-1 in each experimental group, and the differences in any two groups were statistically significant (Group A), low dose IFN-γ intervention group (group L) and high dose group (group B) by the method of immunohistochemistry (IHC) The expression of CysLTl positive cells in the intervention group (H group) were 11.889 ± 2.369, 19.333 ± 3.240, 26.000 ± 5.522 and 33.222 ± 4.493, respectively, with statistical significance (P <0.01) The relative density of CysLT1 mRNA / β-actin in group N, group A, group L and group H were 0.218 ± 0.050,0.323 ± 0.084,0.447 ± 0.083,0.670 ± 0.108 respectively, and the difference between any two groups was statistically significant ( Pa <0.01). Conclusions Both high and low doses of IFN-γ upregulate the expression of CysLT1 in the lung tissue of asthmatic rats. IFN-γ may play an important role in the pathogenesis of asthma by up-regulating the expression of CysLT1.