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目的评价运用SD大鼠建立高脂血症动物模型的效果。方法 24只5周龄健康SD大鼠(雌雄各半)根据体重随机分为正常对照组、高脂模型组,分别饲以基础饲料、高脂饲料。于喂养的第28天、第45天麻醉各组大鼠,经眼球采血,测定血清中血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)和低密度脂蛋白(LDL-C)的变化。结果在造模28天时,与对照组相比,模型组大鼠血清TC、LDL-C水平明显升高(P<0.01),而TG水平降低(P<0.05)。在造模45天时,模型组大鼠血清TC、LDL-C水平升高,与对照组相比差异有统计学意义(P<0.05),血清TG、HDL-C水平与对照组相比差异无统计学意义。造模28天和48天血脂水平相比差异没有统计学意义。结论 SD大鼠经喂养高脂饲料28天后可以成功建立较理想的高脂血症动物模型。
Objective To evaluate the effect of using SD rats to establish hyperlipemia animal model. Methods Twenty-four healthy 5-week-old SD rats (half male and female) were randomly divided into normal control group and high-fat model group according to body weight, and were fed with basic diet and high-fat diet respectively. On the 28th day and the 45th day of feeding, the rats in each group were anesthetized and blood was collected by eyeball to measure the content of serum total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C) and low density Changes in lipoprotein (LDL-C). Results Compared with the control group, the levels of TC and LDL-C in model group were significantly increased (P <0.01) and the level of TG was decreased (P <0.05) at 28 days. At the 45th day after modeling, the levels of serum TC and LDL-C in the untreated group increased significantly compared with those in the control group (P <0.05). The levels of serum TG and HDL-C in the model group were significantly lower than those in the control group Statistical significance. There was no significant difference in lipid levels between the 28th day and the 48th day after modeling. Conclusion SD rats can successfully establish the ideal hyperlipemia animal model by feeding high fat diet for 28 days.