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采用酶联免疫法和茎基部取样的方式,以强宿根甘蔗品种‘新台糖22号’为对照,通过田间试验研究了同一组合(‘粤糖97-20’×ROC25)杂交后代分离产生的强宿根甘蔗品(种)系(‘云蔗06-407’、‘云蔗06-415’)和弱宿根甘蔗品(种)系(‘云蔗06-408’、‘云蔗06-416’)宿根萌发期内源激素含量的变化与甘蔗宿根性的关系。结果显示:(1)宿根萌发过程中,强宿根与弱宿根甘蔗茎基部IAA含量、ZR含量和ZR/IAA比值的变化波动大,但差异不显著。(2)强宿根与弱宿根甘蔗茎基部GA3和ABA含量、IAA/ABA和GA3/ABA比值的变化差异较大,其中ABA含量、IAA/ABA和GA3/ABA比值的差异显著,是与甘蔗宿根性关系密切的3个重要参数。(3)强宿根甘蔗ABA含量高于弱宿根甘蔗,而其IAA/ABA和GA3/ABA比值小于弱宿根甘蔗,且IAA/ABA和GA3/ABA比值随甘蔗宿根性的减弱而增大。研究认为,在宿根萌发期,甘蔗宿根性越强,ABA含量越高,IAA/ABA和GA3/ABA比值越小;而这3个重要参数均与ABA有关,进行甘蔗宿根性评价应着重参考ABA含量,同时结合IAA/ABA和GA3/ABA比值再进行深入分析鉴定。
Using enzyme-linked immunosorbent assay and stem-base sampling method, strong sugarcane variety Xingtai 22 was used as a control to study the effect of isolating offspring of the same combination (’Yue Tang 97-20’ × ROC25) The roots of sugarcane varieties (’Yunzhan 06-407’, ’Yunzhan 06-415’) and weaker roots (’Yunzhan 06-408’, ’Yunzhan 06-416’) Relationship between changes of endogenous hormones and rooting ability of sugarcane in germinating stage of. The results showed that: (1) The changes of IAA content, ZR content and ZR / IAA ratio in the roots of strong and weak-rooted sugarcane swarmed during the period of perennial germination, but the difference was not significant. (2) There were significant differences in the contents of GA3 and ABA, IAA / ABA and GA3 / ABA between the roots of strong and weak dormant sugarcane, and the differences of ABA content, IAA / ABA and GA3 / ABA were significant, Root close three important parameters. (3) ABA content of strong sugarcane was higher than that of weak sugarcane, while IAA / ABA and GA3 / ABA ratios of weak sugarcane were lower than that of weak sugarcane, and the ratios of IAA / ABA and GA3 / ABA increased with the decrease of sugarcane perenniality. The results showed that the higher the ABA content was, the lower the ratio of IAA / ABA and GA3 / ABA was. The three important parameters were all related to ABA. For the evaluation of sugarcane abortion, ABA Content, combined with the ratio of IAA / ABA and GA3 / ABA and then in-depth analysis and identification.