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目的:将链亲和素标记的粒细胞巨噬细胞集落刺激因子(SA-GM CSF)原位锚定成瘤小鼠膀胱,探求该新疗法的可行性和有效性。方法:选择雌性C57BL/6小鼠50只,随机均分成5组,组1到组4建立原位表浅膀胱癌模型后,按照处理的不同又分为PBS组、GM CSF组、SA-GFP组和SA-GM-CSF组,分别给予相应治疗;6次治疗后,SA-GM-CSF组和组5(对照组)接受第二次肿瘤细胞攻击;观察所有小鼠的一般情况、肿瘤情况和生存期,并对死亡小鼠进行解剖,留取器官组织做病理切片或免疫组织化学染色。结果:SA-GM CSF组与其它各组比较,肿瘤出现的时间晚,肿瘤生长速度慢,小鼠生存期长(P_(max)=0.023,P<0.05);MB49细胞二次攻击后,SAGM-CSF组与对照组相比,生存期差异有显著统计学意义(x~2=9.551.P=0.002)。结论:SA GM-CSF原位锚定于雌性C57BL/6成瘤小鼠膀胱黏膜,有效抑制了膀胱肿瘤的生长,延长了小鼠的生存时间,并且能抵抗同源肿瘤的再次攻击,提示可能诱导了小鼠针对MB49的免疫保护作用;SA-GM-CSF原位锚定治疗比单纯细胞因子灌注法疗效更好。
OBJECTIVE: To anchor streptavidin-labeled granulocyte-macrophage colony-stimulating factor (SA-GM CSF) into the bladder of tumor-bearing mice in situ and explore the feasibility and effectiveness of the new therapy. Methods: Fifty female C57BL / 6 mice were randomly divided into 5 groups. Groups 1 to 4 were divided into four groups: PBS group, GM CSF group, SA-GFP group SA-GM-CSF group and SA-GM-CSF group were given corresponding treatment; after 6 treatments, SA-GM-CSF group and group 5 (control group) received the second tumor cell challenge; the general situation of all mice, And survival, and the dead mice were dissected, organ histology was taken for histological examination or immunohistochemical staining. Results: Compared with other groups, the SA-GM CSF group showed a late appearance of tumor and a slow growth of the tumor. The survival time of the mice in the SA-GM CSF group was longer (P max = 0.023, P <0.05). After the second challenge, the SAGM There was a significant difference in survival between the CSF group and the control group (x ~ 2 = 9.551.P = 0.002). CONCLUSIONS: SA-GM-CSF is anchored in situ into the bladder mucosa of female C57BL / 6 mice and effectively inhibits the growth of bladder tumors, prolongs the survival time of mice and resists the re-attack of homologous tumors, suggesting that it may Induced mouse immunoprotection against MB49; SA-GM-CSF in situ anchoring treatment is better than cytokine alone.