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目的采用气相色谱/质谱法检测人体血液和肾、肝组织中的丙泊酚。方法心血和肾、肝组织中的丙泊酚经环己烷提取,采用气相色谱/质谱法,丙泊酚选择m/z 163、178,内标百里香酚选择m/z 150、135进行测定;并考察方法专属性、线性范围、定量线与检出限、回收率与精密度。结果丙泊酚的保留时间为8.17min,与内标百里香酚分离良好;其在心血和肾、肝组织中的检测浓度线性关系良好,r值均大于0.992,最低检测质量浓度分别为0.05μg/mL、0.10μg/g及0.05μg/g,方法回收率90%～110%,日内、日间RSD均小于7%。结论本文方法简便、灵敏、快速,其准确度、精密度和回收率均可满足生物检材中丙泊酚浓度的测定,可在相关研究及实践中选用。
Objective To detect propofol in human blood, kidney and liver by gas chromatography / mass spectrometry. Methods Propofol was extracted from blood and kidneys and liver tissues by cyclohexane and determined by gas chromatography / mass spectrometry, m / z 163,178 with propofol and m / z 150,135 with internal standard thymol. The method specificity, linear range, quantitation line and detection limit, recovery and precision were also investigated. Results The retention time of propofol was 8.17min, which was well separated from the internal standard thymol. The linearity of the measured concentration of propofol was good in both heart and kidney and liver tissues, the r values were all greater than 0.992, the minimum detectable concentrations were 0.05μg / mL, 0.10μg / g and 0.05μg / g respectively. The recovery rate was 90% -110%. The intra-day and inter-day RSD were less than 7%. Conclusions This method is simple, sensitive and rapid, and its accuracy, precision and recovery can meet the requirements of propofol concentration in biological samples. It can be used in related research and practice.