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目的研究IGF-Ⅰ在先天性膈疝胎肺上的表达及探讨其在CDH肺发育不良中的作用。方法采用免疫组化和图像分析方法检测IGF-Ⅰ在胎肺中的表达及相对含量。结果IGF-Ⅰ在对照组表达微弱或缺如,在实验组明显表达于细支气管上皮细胞的胞浆及部分神经丛,其相对含量显著高于对照组。IGF-Ⅰ与平均肺泡面积具有显著的负相关。结论CDH肺发育不良可能是原发性;IGF-Ⅰ的表达增高,具有组织特异性;IGF-Ⅰ与肺泡面积呈负的直线相关性。
Objective To study the expression of IGF-Ⅰ in fetal lung of congenital diaphragmatic hernia and its role in CDH pulmonary dysplasia. Methods Immunohistochemistry and image analysis were used to detect the expression and relative content of IGF-Ⅰ in fetal lung. Results The expression of IGF-Ⅰ was weak or absent in the control group, which was significantly higher in the cytoplasm and part of plexus of the bronchiolar epithelial cells in the experimental group than in the control group. IGF-Ⅰ and the average alveolar area has a significant negative correlation. Conclusions CDH dysplasia may be primary; IGF-Ⅰ expression is increased and tissue-specific; IGF-Ⅰ is negatively correlated with alveolar area.