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以260份花生材料为试材,采用不同的遗传距离构建吉林省花生初级核心种质。在50%的取样比例下,采用数量性状参数均值差异百分率、方差差异百分率、极差符合率、变异系数变化率4个指标评价不同方法构建的初级核心种质的可行性和有效性。最终选出一种合适的方法构建花生初级核心种质,并利用等位变异数、有效等位变异数、Shannon-Weaver指数、Nei期望杂合度等SSR标记相关参数进行验证。研究结果表明:基于欧式距离,采用优先取样法结合最短距离法进行聚类分析,构建包含128个样品的花生核心种质。采用SSR标记参数进行验证,表明128份初级核心种质可以代表原种质80%的遗传信息,较好地代表了原种质的遗传多样性。
With 260 peanut materials as tested material, the primary core collection of peanut in Jilin Province was constructed with different genetic distance. Under the sampling ratio of 50%, the feasibility and validity of the primary core collection constructed by different methods were evaluated by using four indicators of mean value difference, variance difference percentage, very poor coincidence rate and coefficient of variation of variation coefficient. Finally, a suitable method was selected to construct the primary core collection of peanut. The SSR markers such as allele number, effective allele number, Shannon-Weaver index and Nei expected heterozygosity were used for validation. The results showed that based on the Euclidean distance, clustering analysis was carried out by using the method of prior sampling and the shortest distance method to construct 128 core samples of peanut germplasm. The SSR marker parameters were used for validation, indicating that 128 primary core germplasm can represent 80% of the genetic information of the original germplasm, better representative of the genetic diversity of the original germplasm.