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目的对肠道病毒71型(EV71)VP1基因编码衣壳蛋白进行系统结构与功能预测,为制备EV71疫苗和诊断抗原的研究提供理论基础。方法基于编号为1404-Luan(CHN)-08的EV71六安地区分离株VP1基因编码蛋白的氨基酸序列,应用ProtParam、SigalP、TMPRED、Sosui、SOPMA、Phyre、Bepipred等生物信息学方法预测其信号肽、跨膜区、疏水性、二级结构、三级结构等性质和潜在的B细胞表位。结果 EV71六安地区分离株VP1基因编码蛋白没有信号肽,无跨膜区,是一个亲水性蛋白;VP1蛋白二级结构以无规则卷曲和β-片层为主,含有少量的α-螺旋,少见β-转角;VP1的三级结构中4～121氨基酸部分在蛋白表面形成一环状结构(VPl GH loop);EV71 VP1潜在的B细胞表位共有6个可能的抗原表位。其中,预测分值最高的线性表位区域位于157～177位氨基酸残基。结论成功预测了EV71六安地区分离株VP1二级结构、三级结构和B细胞表位,为制备EV71基因工程疫苗和诊断抗原的开发提供了理论基础。
Objective To predict the structure and function of the capsid protein of the VP1 gene of enterovirus 71 (EV71), and to provide a theoretical basis for the study of EV71 vaccine and diagnosis of antigen. Methods Based on the amino acid sequence of the VP1 gene in the EV71 isolate of EV71 numbered 1404-Luan (CHN) -08, the signal peptides were predicted by bioinformatics methods such as ProtParam, SigalP, TMPRED, Sosui, SOPMA, Phyre and Bepipred , Transmembrane region, hydrophobicity, secondary structure, tertiary structure and other properties and potential B cell epitopes. Results The VP1 gene of EV71 isolate had no signal peptide and no transmembrane domain and was a hydrophilic protein. The secondary structure of VP1 protein was mainly random coil and β-sheet with a small amount of α-helix , With a rare β-turn. The 4 to 121 amino acids in the tertiary structure of VP1 form a loop (VP1 GH loop) on the protein surface. There are 6 possible epitopes in the potential B cell epitope of EV71 VP1. Among them, the region of linear epitope with the highest predicted score is located at amino acid residues 157-177. Conclusion The secondary structure, tertiary structure and B cell epitope of VP1 in the EV71 Lu’an area were successfully predicted, which provided a theoretical basis for the development of the EV71 genetically engineered vaccine and the development of diagnostic antigens.