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目的评价中国疾病预防控制中心(CDC)病毒病预防控制所国家脊髓灰质炎(脊灰)实验室所用细胞系对脊灰病毒的敏感性,制备国家脊灰实验室质量控制株(China Sabin Master Reference,CSMR)和用于脊灰实验室网络的中国Sabin参考株(China Sabin Test Reference Strain;CSTRS),提供给省级CDC脊灰实验室用于其对所使用的RD-A、L20B细胞系的敏感性评价。方法96孔微量培养板滴定法。结果国家脊灰实验室CSMR 3次独立的细胞敏感性实验结果的滴度波动在±0.5log10,同时用英国国家生物标准品与控制样品研究所(NIBSC)提供的已知滴度Sabin脊灰病毒参考株(NIBSC原始株)作平行对照,NIBSC原始株3次滴度结果与其本身提供的参考值相比较,其滴度波动也均在±0.5log10。结论通过NIBSC原始株3次滴度结果作平行对照,证实中国CSMS结果符合实验要求,国家脊灰实验室的内部CSMR结果有效;从而制定了国家脊灰实验室质量控制参考株的滴度参考值,表明国家脊灰实验室所用细胞系的敏感性未下降,实验程序无误。CSTRS可作为标准株分发到省级CDC脊灰实验室用于进行细胞系敏感性实验。
Objective To evaluate the sensitivity to poliovirus of the cell lines used in the national poliovirus (polio) laboratory of the Chinese Center for Disease Control and Prevention (CDC) virus prevention and control, and to prepare the China Sabin Master Reference , CSMR) and the China Sabin Test Reference Strain (CSTRS) for the polio laboratory network were provided to provincial CDC polio laboratories for their use in the RD-A, L20B cell lines used Sensitivity evaluation. Methods 96-well microplate titration. Results The results of three independent cell sensitivity tests of CSMR fluctuated within ± 0.5log10 in the national polio laboratory and the titer of the known Sabin poliovirus in the National Institute for Bioscience and Controlled Research (NIBSC) Reference strains (NIBSC original strain) as a parallel control, NIBSC original strain three titers results and its own reference value compared to the titers were also fluctuations of ± 0.5log10. Conclusion The results of three titers of original strain of NIBSC were used as a parallel control, which confirmed that the results of CSMS in China met the requirements of the experiment and the internal CSMR results of the national pollen laboratory were effective. Thus, the reference value of the quality control reference strain of the national polio laboratory , Indicating that the national polio laboratory cell lines used in the sensitivity did not decline, experimental procedures correct. CSTRS can be distributed as a standard strain to provincial CDC polio laboratories for cell line sensitivity experiments.