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目的:主要探讨乳腺浸润性癌组织中PICK-1蛋白的表达与该肿瘤临床病理、其他生物学指标以及患者预后的相关性。方法:采用组织芯片免疫组织化学染色方法检测496例乳腺浸润性癌组织中PICK-1、ER、PR、HER2/neu、TlMP-1等蛋白的表达情况及表达间的相关性,以及PICK-1蛋白在不同分子亚型乳腺癌中的表达情况及对预后的影响。结果:PICK-1蛋白在乳腺癌组织中的表达率为27.22%,肿瘤细胞中PICK-1的高表达与淋巴结受累和高组织学分级相关(P均<0.001),且与HER2/neu表达相关(P<0.001),而与雌孕激素受体状态无关(P>0.05),同时该蛋白的表达与TIMP-1表达呈正相关(P=0.002)。PICK-1蛋白表达与患者总生存期缩短相关,分层分析显示PICK-1仅对淋巴结阳性患者的预后有影响,同时对HER2/neu不同状态患者的影响不同,其中对三阴性乳腺癌(HR-HER-)预后影响最大。单因素分析显示乳腺肿瘤直径、组织学分级、淋巴结状态、HR状态、HER2/neu表达、TIMP-1表达、肿瘤细胞表达PICK-1均是有意义的预后指标;但多因素分析显示仅肿瘤直径、组织学分级、淋巴结状态、HER2/neu袁达和PICK-1表达是独立的预后因素。结论:PICK-1蛋白与浸润性乳腺癌的侵袭和转移特性相关,同时有可能作为患者预后不良的指标,尤其是对于三阴性乳腺癌的预后意义更为显著。
Objective: To investigate the relationship between the expression of PICK-1 protein and the clinicopathological features, other biological indexes and the prognosis of patients with invasive breast cancer. Methods: The expressions of PICK-1, ER, PR, HER2 / neu and TlMP-1 in 496 cases of invasive breast cancer were detected by immunohistochemical staining. The correlation between the expressions of PICK-1, Protein Expression in Different Molecular Subtypes of Breast Cancer and Its Impact on Prognosis. Results: The positive rate of PICK-1 protein in breast cancer was 27.22%. The high expression of PICK-1 in tumor cells correlated with lymph node involvement and histological grade (all P <0.001), and was correlated with HER2 / neu expression (P <0.001), but not with estrogen and progesterone receptor status (P> 0.05). Meanwhile, the expression of TIMP-1 and TIMP-1 protein were positively correlated (P = 0.002). PICK-1 protein expression was correlated with shortened overall survival. Stratified analysis showed that PICK-1 only affected the prognosis of lymph node-positive patients and had different effects on patients with different status of HER2 / neu. Among them, triple-negative breast cancer -HER-) The prognosis is most affected. Univariate analysis showed that tumor diameter, histological grade, lymph node status, HR status, HER2 / neu expression, TIMP-1 expression and PICK-1 expression in tumor cells were all significant prognostic indicators; however, multivariate analysis showed only tumor diameter , Histological grade, lymph node status, HER2 / neu expression and PICK-1 expression were independent prognostic factors. Conclusion: PICK-1 protein is associated with the invasion and metastasis of invasive breast cancer, and may be used as an indicator of poor prognosis in patients, especially for the prognosis of triple negative breast cancer.